舒氧康对急性肺损伤家兔肺组织肿瘤坏死因子-αmRNA表达的影响

目的:探讨舒氧康对急性肺损伤(ALI)家兔肺组织肿瘤坏死因子-α(TNF-α)mRNA表达的影响及其对肺损伤的治疗作用。方法:将45只家兔随机分为对照组(A组)、肺损伤组(B组)和舒氧康组(C组),每组15只,B和C组采用特制多功能撞击机制成ALI模型,C组经耳缘静脉给予舒氧康静滴[0.3mL/(kg·min)],分别在损伤前及损伤后2、4、6h采血测定TNF-α水平,6h后处死动物,取肺组织测定肺组织TNF-α水平、TNF-αmRNA表达、肺水含量、肺体质量比值及观察病理变化。结果:与A组相比,B组损伤后肺组织及血浆TNF-α水平、肺组织TNF-αmRNA表达显著升高(P<0.05),PaO2显著降低(P<0.05),肺水含量及肺体质量比值显著增高(P<0.05),镜下见肺间质、肺泡水肿,大量炎细胞浸润。C组经舒氧康治疗后,与B组比较,肺组织及血浆TNF-α水平、肺组织TNF-αmRNA表达降低(P<0.05),PaO2明显升高(P<0.05),肺水含量及肺体质量比值减少,肺水肿减轻。结论:舒氧康能抑制ALI家兔肺组织TNF-αmRNA的表达,降低肺组织...更多和血浆TNF-α水平,减轻肺组织的病理损害从而治疗ALI。

Effects of oxygenator on TNF-α mRNA expression in the lung tissue of rabbits with acute lung injury

Objective To study the effects of oxygenator on tumor necrosis factor-α (TNF-α) mRNA expression in the lung tissue and the therapeutic role of oxygenator in acute lung injury (ALI) in rabbits. Methods Forty-five rabbits were randomized to control group (group A), ALI group (group B), or oxygenator treatment group (group C), 15 for each group. The model of ALI was established in groups B and C using a specially made impactor and an intravenous oxgenator of 0.3 mL/(kg·min) was administered in group C. Plasma TNF-α levels at baseline and 2,4,and 6 h after ALI were detected. The rabbits were then executed and TNF-α levels and TNF-α mRNA expression in the lung tissue,lung water content, and lung weight/body weight (LW/BW) ratio were measured, and pathological changes in the lung tissue were microscopically observed. Results As compared with group A, plasma TNF-α levels and TNF-α levels and TNF-α mRNA expression in the lung tissue and plasma were significantly increased in group B(P < 0.05), so were lung water content and LW/BW ratio(P < 0.05), but PaO2 was significantly decreased (P < 0.05). Interstitial and alveolar edema and massive inflammatory cell infiltration were observed. However,as compared with group B,plasma TNF-α levels and TNF-α levels and TNF-α mRNA expression in the lung tissue and plasma were markedly declined in group C(P < 0.05), so were lung water content and LW/BW ratio (P < 0.05), while PaO2 was notably elevated (P < 0.05), and pulmonary edema was alleviated. Conclusion Oxygenator inhibits TNF-α mRNA expression in the lung tissue, lowers TNF-α levels in the lung tissue and plasma, alleviates pathological changes, which is an effective therapy for ALI.