三维建模的猫听皮质定位与主要分区的研究

目的研究猫听皮质的定位与主要分区的三维可视化。方法利用生物素葡聚糖胺(BDA)进行顺行追踪,建立猫听皮质区域的原始数据库,通过3D软件Amira进行重构。结果建立了猫大脑半球及其听皮质区域的数据库,将听皮质分为AⅠ、AAF、P、VP、AⅡ五区,重建了大脑半球及其主要听皮质分区的数字化解剖模型。结论将组织学技术与计算机图像处理技术相结合,可以从三维的角度来研究和重建听皮质的分区结构。

The localization and Capital Subdivisions of the Auditory Cortex in Cat Based on the Three-dimensional Reconstruction

Objective To outline the three-dimensional anatomic pictures of the cat auditory cortex subdivisions. Methods Anterograde tracer-biotinylated dextran amines(BDA)was injected into the medial geniculate body of the cat,then the original data-base of the auditory subdivisions was built by photographing TMB-processed coronal slices.In the end, three-dimensional software of Amira was introduced to reconstruct auditory subdivisions.Result The data-base of the hemisphere and auditory cortex subdivisions of the eat was obtained.The result of the anterograde tracing showed that the capital auditory regions of the cat cerebral cortex comprise five different areas:AI、AAF、P、VP and AⅡ.Finally,we reconstructed the three-dimensional model of the hemisphere and auditory cortex region.Conclusion Combining histological technique and computer technology,we are allowed to conduct researches on precise and three-dimensional subdivision of the cat auditory cortex.