iNOS在皮肤肿瘤中的表达及其意义

目的:检测诱导型一氧化氮合酶(iNOS)在皮肤良恶性肿瘤中的表达。方法:采用免疫组织化学技术检测25例脂溢性角化病、25例光线性角化病、25例基底细胞癌、30例鳞状细胞癌(I级13例,II-III级17例)、10例正常皮肤组织中iNOS的表达。结果:2例(8.00%)脂溢性角化病、13例(52.00%)光线性角化病、11例(44.00%)基底细胞癌、22例(73.33%)鳞状细胞癌中iNOS呈阳性表达,正常皮肤表达均为阴性。iNOS在鳞状细胞癌、基底细胞癌及光线性角化病中的阳性率明显高于脂溢性角化病组(P<0.01),鳞状细胞癌组与光线性角化病组间无明显差别(P>0.05),与其他各组间差异显著(P<0.01或P<0.05),II III级鳞状细胞癌iNOS表达明显高于I级鳞状细胞癌(P<0.05)。结论:皮肤肿瘤中存在iNOS的表达,其合成的一氧化氮可能在皮肤的癌前病变及恶性肿瘤的发生、发展中起到一定的作用。其表达可能有助于皮肤肿瘤恶性度及预后的判断。

The expression of inducible nitric oxide synthetase (iNOS) and its significance in cutaneous tumors

Objective:To determine the expression of inducible nitric oxide synethetase (iNOS) in innocent and malignant cutaneous tumors. Methods: The expression of iNOS in tissue samples was measured by immunohistochemistry method. Results: The iNOS was detected in 2 of 25 seborrheic keratosis (SK), 13 of 25 actinc keratosis (AK), 11 of 25 basal cell carcinoma (BCC) and 22 of 30 squamous cell carcinoma (SCC). The expression of iNOS in AK, BCC and SCC significantly higher than that in SK (P<0.01). There was no significant difference between SCC and AK (P>0.05), but in SCC the expression was higher than other diseases (P<0.01 or P<0.05). In SCC grade-II III the expression was significantly higher than SCC grade-I (P<0.05).Conclusion: iNOS expression exists in cutaneous tumor, which may be involved in the development of premalignant and malignant cutaneous tumors by generating NO. It can be used to judge malignant degree and to predict prognosis.