CD4+CD25+调控T细胞对小鼠胃癌的影响

目的在体研究CD4^＋ CD25^＋调控T（Treg）细胞对小鼠胃癌的影响。方法检测荷瘤小鼠不同时相点Treg细胞比例的变化。并应用免疫磁珠法（MACS法）分选、纯化小鼠Treg细胞：以不同剂量的Treg细胞及Treg细胞拈抗剂Anti-GITR注入荷瘤小鼠，3周后观察小鼠移植瘤的体积及胃癌细胞凋亡率的变化。结果正常小鼠脾脏Treg细胞为（3．86±0．07）％，在小鼠胃癌模型中Treg细胞比例逐渐升高，第3周时为（4．12±0．13）％，明显高于对照组（P=0．015）。在体应用Treg细胞达到2．0×10^5时，小鼠移植瘤的体积明显增大（P=0．013），胃癌细胞凋亡率明显降低（P=0．012）。当应用Treg细胞拮抗剂Anti-GITR剂量为100μg时，种植瘤的体积明显小于对照组（P=0．008），其凋亡比例明显高于对照组（P=0．021）。结论在胃癌小鼠模型中，随着胃癌的进展，Treg细胞的比例有增高的趋势。应用Treg细胞能促进小鼠胃癌生长。降低胃癌细胞的凋亡；相反、应用Treg细胞拈抗剂Anti-GITR可以提高小鼠机体的抗胃癌作用。

Influence of CD4+ and CD25+ T regulatory cell on mouse bearing gastric tumor in vivo

OBJECTIVE: To investigate the influence of CD4+ CD25+ regulatory T cells(Treg cells) on mouse gastric cancer. METHODS: Treg cell in mouse spleen bearing gastric tumor was tested in different time points. Magic cell sorting(MACS) method was used to purify mouse Treg cells and the Treg cells were injected into mouse bearing gastric tumor with different dosage. After 3 weeks, the tumor size and tumor cell apoptosis rate were measured. RESULTS: Treg existed in normal mouse spleen with a rate of (3.86+/-0.07)%. In tumor model this percentage increased gradually and was (4.12+/-0.13)% after 3 weeks, which was significantly higher than that in control. When Treg cell applied in mouse reached 2.0 x 10(5), the tumor size enlarged significantly(P=0.013) and tumor cell apoptosis rate decreased significantly (P=0.012). CONCLUSIONS: Treg cell is associated with gastric cancer progress in mouse tumor model. Treg cell can promote gastric cancer growth and decrease tumor apoptosis. The anti- Treg GITR can improve anti- tumor effects.