| 琼脂扩散盒法检测抗癌药物对人癌克隆原细胞集落形成的影响 |
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| 引用本文： | 李威威,彭祥鄂,叶雨文.琼脂扩散盒法检测抗癌药物对人癌克隆原细胞集落形成的影响[J].中南大学学报(医学版),1986(3). |
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| 作者姓名： | 李威威 彭祥鄂 叶雨文 |
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| 作者单位： | 湖南医学院药理学研究室 研究生
(李威威)，湖南医学院药理学研究室 导师
(彭祥鄂)，湖南医学院药理学研究室 导师(叶雨文) |
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| 摘 要： | 本文采用改良琼脂扩散盒法测定冬凌草甲素、高三尖杉酯碱及三种已知抗癌药物环磷酰胺、5-氟脲嘧啶和顺铂对人癌克隆原细胞集落形成的影响。初步显示高三尖杉酯碱对肺癌和乳腺癌、冬凌草甲素对肝癌可能有抑制作用。试验结果表明,采用本法进行抗癌药物活性测定较为稳定可靠,对需经代谢活化的药物也能作出合适的效果评价。
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| 关 键 词： | 肿瘤干细胞测定 人类 抗肿瘤药 药物作用 琼脂扩散盒 冬凌草甲素 高三尖杉脂碱 |
OBSERVATION ON COLONY FORMATION OF HUMAN TUMOR CLONOGENIC CELLS BY USING MODIFIED AGAR DIFFUSION CHAMBER TECHNIQUE |
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| Li Weiwei Peng Xiang-e Ye Yuwen Research Section of Pharmacology,Hunan Medical College.OBSERVATION ON COLONY FORMATION OF HUMAN TUMOR CLONOGENIC CELLS BY USING MODIFIED AGAR DIFFUSION CHAMBER TECHNIQUE[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),1986(3). |
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| Authors: | Li Weiwei Peng Xiang-e Ye Yuwen Research Section of Pharmacology Hunan Medical College |
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| Abstract: | A modified agar diffusion chamber technique was utilized to grow colonies from 51 fresh specimens of human solid tumors. Effects of Rubescensine A, Homo-harringtonine and three known anticancer drugs on human tumor clonogenic cells were assayed. Preliminary results indicated that Homoharringtonine had antitumor activity against human breast cancer(l/3) and lung cancer(l/4). Antitumor activity was also seen for Rubescensine A against human hepatic cancer (1/1). Otherwise, different sensitivity of human tumor clonogenic cells to drugs was observed among tumors with the same histological type. Results also showed that this technique was reliable in vivo drug sensitivity assay of human tumor clonogenic cells,and drugs in which biotransformation is required for their activities also can be evaluated. |
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| Keywords: | Tumor stem cell assay Human Antineoplastic agents Drug effects Agar diffusion chamber Rubescensine A Homoharringtonine |
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