多聚酶链反应临床应用970例分析

目的探讨多聚酶链反应（PCR）检测患者淋球菌（NG）、人乳头病毒（HPV）、结核杆菌（TB）、乙肝病毒（HBV）、肺炎支原体（PM）、疱疹病毒（HSV）、非淋菌性尿道炎（NGU）的沙眼衣原体（CT）和解脲支原体（MU）的临床意义。方法采用FQ-PCR方法检测临床上拟诊断为淋球菌感染（淋病）、尖锐湿疣、结核、支原体肺炎、非淋菌性尿道炎患者的沙眼衣原体（CT）和解脲支原体（MU）的970例患者，判断其阳性率。结果NG阳性率38．2％（240／627），HPV阳性率66．O％（70／106），TB阳性率27．7％（20／72），HBV阳性率35．1％（33／94），PM阳性率33．3％（2／6），HSV阳性率20．O％（1／5），MU阳性率12．0％（4／33），CT阳性率22．0％（6／27）。结论FQ-PCR方法具有简便、快速、准确、特异性强等优点，可应用于常见细菌、病毒、衣原体、支原体等疾病的诊断、治疗及疗效观察。

Clinical Application of PCR in 970 Cases

Objective To discuss the clinical significances of using polymerase chain reaction (PCR) to exam Neisseria gonorrhoeae (NG),human papilloma virus (HPV),tuberculosis bacillus (TB),hepatitis B virus (HBV),pneumonia mycoplasma (PM),herpes simplex virus (HSV),non-gonococcus urethritis (NGU) Chlamydi trachomatis (CT) and Mycoplasma urealytium (MU). Methods By using the FQ-PCR method to examine 970 clinical examples that had been diagnosed as gonococcus infection (gonorrhea),incisive condyloma,tuberculosis,mycoplasma pneumonia,non-gonococcus urethritis patient's blister Chlamydia trachomatis (CT) conciliatory Mycoplasma urealytium (MU). Results The positive rate of NG was 38.2%(240/627),of HPV 66.0%(70/106),of TB 27.7% (20/72),of HBV 35.1% (33/94),of PM 33.3%(2/6),of HSV 20.0% (1/5),of MU 12.0% (4/33),of CT 22.0%(6/27).Conclusion FQ-PCR is easy,rapid,accurate and can be used to examine the common infectious diseases caused by bacterium,virus,chlamydia,mycoplasma and so on.