Detection of Chlamydia pneumoniae DNA in atheromatous tissues by polymerase chain reaction |
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Authors: | Nadrchal R Makristathis A Apfalter P Rotter M Trubel W Huk I Polterauer P Hirschl A M |
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Affiliation: | Klinische Abteilung für Klinische Mikrobiologie des Klinischen Institutes für Hygiene, Vienna, Austria. |
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Abstract: | Chlamydia pneumoniae is an important human respiratory pathogen. Recently, seroepidemiological data and the demonstration of chlamydial DNA or antigen within parts of atherosclerotic lesions have suggested a causal association between chlamydial infections and atherosclerosis. As the results obtained by different groups show considerable variations, we provide further data based on a highly specific and sensitive nested PCR method. Positivity was confirmed by nonradioactive hybridization with a specific probe, and sensitivity was determined by titration experiments. C. pneumoniae DNA was detected in 8/29 (28%) of carotid artery samples and 3/14 (21%) of aortic aneurysms. |
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