儿童慢性乙型肝炎病毒携带者T细胞亚群的研究

目的研究儿童慢性乙型肝炎病毒（HBV）携带者（ASC）的T细胞亚群状态。方法设对照组（n=32）及ASC组（n=104），ASC组中又分别根据HBV—DNA及HBeAg的定性分为HBV—DNA阳性组（n=62）及阴性组（n=42）和HBeAg阳性组（n=56）及阴性组（n=48）。血清HBV标志物和HBV-DNA分别经酶联免疫吸附试验（EUSA）和基因扩增技术聚合酶链反应（PCR）法检测，T细胞亚群采用碱性磷酸酶-抗碱性磷酸（APAAP）免疫组化染色法检测。结果ASC组CD3、CD4、CD4/CD8明显低于对照组（P〈0．05或〈0．01），CD8明显高于对照组（P〈0．01）；其中HBV—DNA阳性组CD．、CDJCD8明显低于HBV—DNA阴性组（P〈0．05或〈0．01），CD8明显高于HBV—DNA阴性组（P〈0．01）；HBeAg阳性组的CD4、CD4/CD8较HBeAg阴性组明显降低（P〈0．05或〈0．01）；CD8明显升高（P〈0．01）。结论儿童ASC存在T细胞亚群功能紊乱，尤其以HBV—DNA阳性及HBeAg阳性者为著。

Study on T-cell subsets of child carriers with chronic hepatitis B-virus

Objective To investigate the T-cell subsets of child carriers with chronic hepatitis B-virus(HBV). Methods One hundred and four child carriers with chronic HBV(ASC group) and healthy children(control group,n=32) were selected. The former were divided into HBV-DNA(+) group (n=62) or HBV-DNA(-) group (n=42) and HBeAg(+) group (n=56) or HBeAg(-) group (n=48) according to HBV-DNA and HBeAg respectively. T-cell subsets in the peripheral blood were measured by APAAP, HBV-signs were measured by ELISA, and HBV-DNA was measured by polymerase chain reaction (PCR). Results The mean concentrations of CD3,CD4,CD4/ CD8 in ASC group were significantly lower than those in control group(P<0.05 or<0.01), the level of CD8 was markedly higher (P<0.01). CD4,CD4/ CD8 in HBV-DNA(+) group were significantly lower than those in HBV-DNA(-) group(P<0.05 or<0.01),CD8 was markedly higher (P<0.01). CD4,CD4/ CD8 in HBeAg(+) group were significantly higher than those in HBeAg(-) group (P<0.05 or<0.01)and CD8 was markedly higher (P<0.01). Conclusion There is deficiency of T-cell subsets in child carries with chronic HBV,and this deficiency is more obvious in HBV-DNA(+) group and HBeAg(+) group.