转录因子RORC2和白细胞介素17的表达与变应性鼻炎症状关系的研究

目的 探讨变应性鼻炎(allergic rhinitis,AR)患者转录因子维甲酸相关孤儿受体(retinoic acid-related orphan receptor variant 2,RORC2)和白细胞介素(interleukin,IL)17的表达与过敏症状的关系.方法 取23例AR患者和16例健康人鼻黏膜、鼻分泌物和静脉血,采用免疫组化、实时荧光定量反转录聚合酶链反应和酶联免疫吸附试验检测其中RORC2和IL-17的表达.对AR患者进行症状评分.采用SPSS 13.0软件对变应性鼻炎组与健康对照组各检测指标进行统计学分析.结果 AR组鼻黏膜中RORC2和IL-17免疫组化染色的阳性细胞率分别为0.17±0.05(-x±s,以下同)和0.72±0.13,高于健康对照组的0.05±0.02和0.27±0.11,差异有统计学意义(t值分别为9.51和11.92,P值均＜0.05).AR组鼻黏膜和外周血RORC2 mRNA的相对表达量为0.063±0.011和0.452±0.031,高于对照组的0.029±0.009和0.239±0.027,差异有统计学意义(t值分别为6.51和3.35,P值均＜0.05).AR组IL-17在鼻黏膜、鼻分泌物和外周血中的水平分别为(70.28±10.69)、(45.32±8.55)、(6.76±1.18)pg/ml,高于对照组的(18.43±8.34)、(6.83±1.31)、(0.74±0.05)pgml,差异有统计学意义(t值分别为7.92、17.66和15.43,P值均＜0.05).AR组鼻过敏症状评分为(9.43±1.27)分,分别与鼻黏膜、外周血RORC2 mRNA的表达以及鼻黏膜、外周血IL-17的表达水平呈正相关(r值分别为0.820、0.746和0.629、0.841,P值均＜0.05).结论 RORC2和IL-17参与了AR的炎性反应,可作为判断病情严重程度的指标之一.

Abstract：

Objective To evaluate the relationship between allergic symptoms and retinoic acid related orphan receptor variant 2 (RORC2) and interleukin(IL) 17 in patients with allergic rhinitis(AR).Methods Blood sample,nasal secretion and nasal mucosa were taken from 23 patients with AR and 16 health individuals.The expression of RORC2 and IL-17 were detected by immunohistochemistry and quantitative real-time fluorescence reverse polymerase chain reaction.The allergic symptoms in patients were graded.Results The rate of positive cells of RORC2 and IL-17 in AR group were 0.17 ±0.05 and 0.72 ±0.13,higher than the 0.05 ± 0.02 and 0.27 ± 0.11 of health controls,the difference was statistically significant(t were 9.51 and 11.92 respectively,all P ＜0.05＝.The expression level of RORC2 mRNA in nasal mucosa and peripheral blood of AR group were 0.063 ± 0.011 and 0.452 ± 0.031,higher than the 0.029 ±0.009 and 0.239 ±0.027 of health controls,the difference was statistically significant (t were 6.51and 3.35 respectively,all P ＜0.05＝.The concentrations of IL-17 in the nasal mucosa,nasal secretions and serum levels of AR group were (70.28 ± 10.69) ,(45.32 ± 8.55) and (6.76 ± 1.18) pg/ml,compared with (18.43 ± 8.34),(6.83 ± 1.31) and (0.74 ±0.05) pg/ml of controls,the difference was statistically significant(t were 7.92,17.66 and 15.43 respectively,all P ＜ 0.05＝.The allergy symptom scores of AR group were 9.43 ± 1.27.There were correlations between the allergic symptom and the expression of RORC2 mRNA and IL-17 in nasal mucosa and peripheral blood (r value were 0.820,0.746,0.629,0.841 respectively,all P ＜ 0.05＝.Conclusion RORC2 and IL-17 involved in the inflammatory response of AR and can be used as an indicator to judge the severity.

Relationship between allergic symptoms and RORC2 and IL-17 in patients with allergic rhinitis

Objective To evaluate the relationship between allergic symptoms and retinoic acid related orphan receptor variant 2 (RORC2) and interleukin(IL) 17 in patients with allergic rhinitis(AR).Methods Blood sample,nasal secretion and nasal mucosa were taken from 23 patients with AR and 16 health individuals.The expression of RORC2 and IL-17 were detected by immunohistochemistry and quantitative real-time fluorescence reverse polymerase chain reaction.The allergic symptoms in patients were graded.Results The rate of positive cells of RORC2 and IL-17 in AR group were 0.17 ±0.05 and 0.72 ±0.13,higher than the 0.05 ± 0.02 and 0.27 ± 0.11 of health controls,the difference was statistically significant(t were 9.51 and 11.92 respectively,all P ＜0.05＝.The expression level of RORC2 mRNA in nasal mucosa and peripheral blood of AR group were 0.063 ± 0.011 and 0.452 ± 0.031,higher than the 0.029 ±0.009 and 0.239 ±0.027 of health controls,the difference was statistically significant (t were 6.51and 3.35 respectively,all P ＜0.05＝.The concentrations of IL-17 in the nasal mucosa,nasal secretions and serum levels of AR group were (70.28 ± 10.69) ,(45.32 ± 8.55) and (6.76 ± 1.18) pg/ml,compared with (18.43 ± 8.34),(6.83 ± 1.31) and (0.74 ±0.05) pg/ml of controls,the difference was statistically significant(t were 7.92,17.66 and 15.43 respectively,all P ＜ 0.05＝.The allergy symptom scores of AR group were 9.43 ± 1.27.There were correlations between the allergic symptom and the expression of RORC2 mRNA and IL-17 in nasal mucosa and peripheral blood (r value were 0.820,0.746,0.629,0.841 respectively,all P ＜ 0.05＝.Conclusion RORC2 and IL-17 involved in the inflammatory response of AR and can be used as an indicator to judge the severity.