Effect of leptin on bone metabolism in rat model of traumatic brain injury and femoral fracture |
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Authors: | WANG Lei YUAN Ji-shan ZHANG Hong-xi DING Hua TANG Xing-guo WEI Yong-zhong |
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Institution: | [1]Department of Orthopedics, Affiliated People's Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China [2]Department of Orthopedics, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China |
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Abstract: | Objective: To observe serum and callus leptin expression within the setting of fracture and traumatic brain injury (TBI).Methods: Atotal of 64 male SD rats were randomized equally into 4 groups: nonoperated group, TBI group, fraeture group, and fracture+TBI group. Rats were sacrificed at 2, 4, 8 and 12 weeks after fracture+TBI. Serum leptin was detected using radioimmunoassay, and callus formation was measured radiologically. Callus leptin was analyzed by immunohistochemistry.Results: Serum ieptin levels in the fracture group, TBI group and combined fracture+TBI group were all significantly increased compared with control group at the 2 week time-point (P<0.05). Serum leptin in the combined fracture +TBI group was significantly higher than that in the fracture and TBI groups at 4 and 8 weeks after injury (P<0. 05).The percentage of leptin-positive cells in the fracture+TBI callus and callus volume were significantly higher than those in the fracture-only group (P<0.01).Conclusions: We demonstrated elevated leptin expression within healing bone especially in the first 8 weeks in a rat model of fracture and TBI. A close association exists between leptin levels and the degree of callus formation in fractures. |
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Keywords: | Leptin Brain injuries Fractures bone |
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