P选择素在原发性高血压伴腔隙性脑梗死患者发病机制中的作用

目的观察血小板活化的标志———P-选择素在原发性高血压伴腔隙性脑梗死患者发病机制中的作用。方法利用FITC标记的抗CD42a单抗(鼠IgG1),PE标记的抗CD62P单抗(鼠IgG1),CY标记的抗CD45单抗(鼠IgG1),FITC标记的鼠IgG1同种型对照免疫球蛋白,PE标记的鼠IgG1同种型对照免疫球蛋白,上述单抗分别标记全血中血小板及白细胞的相关抗原;同时利用流式细胞仪,采取全血三色法流式细胞术方法,检测全血血小板上P-选择素阳性表达:全血标本中分别加入3种荧光标记的单克隆抗体FITC-CD42a,PE-62P及CY-CD45,室温避光反应20min,10g/L多聚甲醛固定10min,用PBS洗2次,上机分析。结果原发性高血压伴腔隙性脑梗死患者血小板P-选择素阳性表达犤(23.0±4.0)%犦明显高于原发性高血压无腔隙性脑梗死患者犤(16.9±1.8%)犦及正常人组犤(5.2±0.7)%犦,三者之间相比差异有显著性意义(t=137.4,28.4,P<0.05)。结论P-选择素在原发性高血压伴腔隙性脑梗死发病中起重要作用P-选择素与血管渐进损伤有关。

Effect of P-selectin in primary hypertension associated with lacunar infarction

AIM:To detect the effect of P-selectin,the marker of platelet activation in primary hypertension associated with lacunar infarction. METHODS:FITC-labeled antiCD42a monoclonal antibody(mice IgG1),PE labeled antiCD62P monoclonal antibody(mice IgG1),CY labeled antiCD45 monoclonal antibody(mice IgG1),FITC labeled mice IgG1 isotype matched immune globulin,PE labeled mice IgG contrast immunoglobulin were used to label corresponding antigens of platelets and leukocytes in whole blood.At the same time, P-selectin on the platelet was detected by the method of three-color cytometery in whole blood;Three monoclonal antibodies with fluorescence labeled, FITC-CD42a, PE -62P and CY-CD45 were added in whole blood specimen and incubated for 20 min without light and under room temperature,over night.Then specimen was fixed with citromint for 10 min and washed with PBS twice. RESULTS:The expression of P selectin in hypertension with lacunar infarction patients(23.0± 4.0)% ] was higher than that in hypertension without lacunar infarction patients(16.9± 1.8)% ],as well as higher than that of in normal persons(5.2± 0.7)% ,t=137.4,28.4,P< 0.05]. CONCLUSION:P-selectin plays an important role in primary hypertension associated with lacunar infarction which is related to the progressive injury of vessels.