虎杖不同配伍对糖尿病肾病大鼠糖、脂代谢及血液流变学指标的影响

目的: 观察虎杖分别与黄芪、益母草配伍对糖尿病肾病模型大鼠糖、脂代谢及血液流变学指标的影响,初步探讨其对糖尿病肾病早期干预作用机制。方法: 运用链脲佐菌素诱发SD大鼠糖尿病肾病(DN)模型。成模大鼠随机分为模型组、开博通组、虎杖-益母草配伍组、虎杖-黄芪配伍组。空白组10只,模型组15只,其余3组每组13只。虎杖与益母草配伍、虎杖与黄芪配伍合煎液灌服剂量为3 g·kg^-1,开博通组灌服博通剂量为6.25×10^-3g·kg^-1,模型组和空白组灌服同体积10 mL·kg^-1的生理盐水,每天给药1次,连续给药8周。以血糖、甘油三酯(TG)、胆固醇(Chol)、高密度脂蛋白胆固醇(HDL-L)、低密度脂蛋白胆固醇(LDL-C)、血流变学等为观察指标。结果: 虎杖-黄芪组、虎杖-益母草组在用药第4,8周能明显降低DN模型大鼠血糖水平(P<0.05),明显降低TG水平(P<0.05),对模型大鼠全血黏度、血浆黏度有明显减轻作用,具有显著性差异。其中,虎杖-益母草组对血浆黏度减轻明显(P<0.01)。结论: 虎杖分别配伍黄芪、益母草①对糖尿病肾病糖、脂代谢及血流变具有调节作用;②可能通过降低血糖、甘油三酯等水平,降低全血与血浆黏度,改善微循环,发挥其对糖尿病肾病干预作用。

Effect of Different Compatibility of Polygoni Cuspidati Rhizoma et Radix on Glucose,Lipid Metabolism and Blood Rheology in Diabetic Nephropathy Rats

Objective: To investigate the effect of the compatibility of Polygoni Cuspidati Rhizoma et Radix and Astragali Radix,Polygoni Cuspidati Rhizoma et Radix and Leonuri Herba on glucose, lipid metabolism and blood rheology in diabetic nephropathy rats. Method: Diabetic nephropathy was induced by streptozotocin(STZ) peritoneal injection with a dose of 60 mg·kg^-1(the normal control group was excluded).Based on fasting blood-glucose(FBG) the rats were randomly divided into 4 groups:model group, Kaibotong group,Polygoni Cuspidati Rhizoma et Radix and Astragali Radix group,Polygoni Cuspidati Rhizoma et Radix and Leonuri Herba group. The drugs were given to rats daily for 8 consecutive weeks. At the end of the 2nd,4th,8th week, the rats were fasted after the last administration and then collected the blood samples,glucose oxidase(GOD) was used to examine the fasting blood glucose, trigluceride(TG), cholesterol(CHO), low-density lipoprotein cholesterol(LDL-C), high-density lipoprotein cholesterol(HDL-C) were also tested, the blood rheology indexes were detected. Result: Compared with the model group, the treatment group could effectively restore the condition of diabetic nephropathy rat, the compatibility of Polygoni Cuspidati Rhizoma et Radix and Astragali Radix,Polygoni Cuspidati Rhizoma et Radix and Leonuri Herba could significantly reduce the levels of blood sugar and blood lipid, the difference was statistically significant (P<0.05 and P<0.01), andcould change the whole blood viscosity and plasma viscosity of the diabetic nephropathy rats, the difference was statistically significant (P<0.05 and P<0.01). Conclusion: Different compatibility of Polygoni Cuspidati Rhizoma et Radix demonstrates hypoglycemic and hypolipidemic effects, and can reduce the whole blood viscosity and plasma viscosity.