高效液相色谱-间接光度检测法测定血清中硫酸奈替米星浓度

 目的：建立血清中硫酸奈替米星的高效液相色谱 间接光度检测法(HPC-IDP)。方法：以烟酰胺为检测剂，庚烷磺酸钠为反离子，固定相Spherisorb C₁₈，流动相为甲醇-PH2的磷酸缓冲液(20∶80)，内含烟酰胺0.4mmol·L^-1，庚烷磺酸钠5mol·L^-1。测定了4例患者单剂量im后的血清浓度并计算药动学参数。结果：线性范围0.5mg·L^-1-15.0mg·L^-1，线性回归方程c=0.855-4.384×10^-6H，r=0.998?回收率平均为93.27%，RSD日内平均为4.95%，日间平均为5.71%。结论：本法操作简便，灵敏度高，适于作为血清中硫酸奈替米星的检测方法。

Determination of netilmicin sulfate in serum by high performance liquid chromatography indirect photometric determination method

OBJECTIVE: To determine the content of netilmicin sulfate in serum by a high performance liquid chromatography indirect photometric determination (HPLC-IPD) method. METHOD: The chromatographic system consisted of Soheisorb C₁₈column and mobile phase of a solution of methyl alcohol a buffer of phosphoric acid (pH=2.0) (20∶80), that contained 0.5 mmol·L^-1of nicotinamide and 0.3 mmol·L^-1of sodium seventhalkyl sulphonate. The detected wave length was 268 nm. The serum simple of 4 patients was determined. RESULTS: The mean recovery of was 93.41% and detection limits was 50 μg·L^-1. CONCLUSION: The method is constant, sensitive, and has a good concentration. It is good for determination of netilmicin sulfate concentration in serum.