| 反相高效液相色谱法测定人血清中丙戊酸钠浓度 |
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| 引用本文： | 何林,余继英,吴正中,孙世明. 反相高效液相色谱法测定人血清中丙戊酸钠浓度[J]. 中国药房, 2002, 13(1): 32-33 |
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| 作者姓名： | 何林 余继英 吴正中 孙世明 |
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| 作者单位： | 四川省人民医院药剂科,成都市，610072 |
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| 摘 要： | 目的 :测定人血清中丙戊酸钠浓度。方法 :以反相高效液相色谱法 ,甲醇 -水(70:30)为流动相 ,检测波长为248nm ;采用衍生化法 ,环己烷羧酸为内标。结果 :丙戊酸钠浓度在14 47～248 0μg/ml范围内呈现良好线性关系。结论 :该方法简便、准确 ,适合于临床血药浓度监测。
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| 关 键 词： | 反相高效液相色谱法 丙戊酸钠 衍生化 |
| 文章编号： | 1001-0408(2002)01-0032-02 |
| 修稿时间： | 2001-06-04 |
Determination of the Concentration of Valproic Acid in Serum With RP- HPLC |
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| HE Lin,YU Jiying,WU Zhengzhong,SUN Shiming. Determination of the Concentration of Valproic Acid in Serum With RP- HPLC[J]. China Pharmacy, 2002, 13(1): 32-33 |
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| Authors: | HE Lin YU Jiying WU Zhengzhong SUN Shiming |
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| Abstract: | OBJECTIVE:To determine the concentration of valproic acid in serum.METHODS:Determination was performed with RP-HPLC with methanol:water(70∶30) as mobile phase,α-bromoacetophenone as deriving agent and cyclohexanecarboxylic acid as internal standard,and detected at wavelength 248nm.RESULTS:The calibrating curve of valproic acid was linear in the range of 14.47～248.0μg/ml.CONCLUSION:The method was convenient,rapid,accurate and suitable for TDM. |
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| Keywords: | RP-HPLC valproic acid derivation |
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