rt-PA在血脑屏障氧糖剥夺体外模型中对神经细胞保护的研究

目的 观察重组组织型纤溶酶原激活剂(rt-PA)在体外血脑屏障氧糖剥夺模型中对神经细胞的影响,探讨rt-PA对脑缺血神经元的保护作用.方法 利用Transwell培养小室为支架,构成以人血管上皮细胞为基础的体外血脑屏障模型;利用厌氧培养箱及无糖培养液,建立体外缺血缺氧模型;在体外缺血缺氧模型基础上,采用组织培养技术,培养神经细胞,并在缺血缺氧条件下,使用MTT法进行检测不同剂量rt-PA对神经细胞活性的影响.结果 分别在氧糖剥夺实验(OGD)及非OGD条件下,在不同rt-PA药物的浓度作用下,低浓度(0.312 5 μg/mL)对非OGD条件下神经细胞的生长有促进作用,在OGD条件下,低浓度(0.625 μg/mL,0.312 5 μg/mL)对神经细胞同样具有保护作用.结论 rt-PA可能对脑缺血后神经细胞存在保护作用,并与rt-PA的剂量相关,在OGD条件下,低剂量rt-PA对神经细胞的保护作用更为显著.

Protective Effects of Recombinant Tissue Plasminogen Activator on Neurons in the Model with Oxygen-Glucose Deprivation in Blood-Brain Barrier

Objective To observe the impact of recombin,and explore the protective effects of recombinant tissue plasminogen activator(rt-PA)on cerebral ischemia.Methods Based on human vascular epithelial cells,blood-brain barrier(BBB)model in vitro was established using Transwell culture chambers as the scaffold.Hypoxia-ischemia model in vitro was established with anaerobic incubator and sugar-free medium.Based on the above models,neurons were cultured using tissue culture techniques.At conditions of ischemia and hypoxia,MTT assay was applied to detect effect of different doses of rt-PA on the activity of neurons.Results The result showed that under oxygen-glucose deprivation(OGD)or non-OGD conditions and under different concentration of rt-PA,a low concentration(0.312 5μg/mL)of rt-PA played a catalytic role in the growth of neurons under non-OGD conditions.At the same time,under OGD condition,low concentrations of rt-PA(0.625μg/mL,0.312 5μg/mL)had a protective effect on neurons.Conclusion rt-PA might have protective effects on neurons after cerebral ischemia,which was related to the dose of rt-PA.Under OGD condition,the protective effects of low-dose rt-PA on neurons were more significant.