| 分光光度法测定大豆磷脂中磷的含量 |
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| 引用本文： | 马金玲,张红军,蹇小兵. 分光光度法测定大豆磷脂中磷的含量[J]. 黑龙江医药科学, 2000, 23(1): 21-21 |
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| 作者姓名： | 马金玲 张红军 蹇小兵 |
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| 作者单位： | 1. 大连弘丰制药厂,116052
2. 佳木斯市晨星制药厂 |
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| 摘 要： | 研究分光光度法测定大豆磷脂中磷的含量。方法：将样品中有机磷酸化合解转化为无机磷酸盐再利用在酸溶液中磷酸盐与钼酸铵及Ｆｉｓｋｅ－ｓｕｂｂａｒｏｗ试剂用生成蓝色的化合物，在６６０ｎｍ处用分光光度法测定。结果：磷的浓度在０．０４ｕｇ／ｍｌ－０．１６ｕｇ／ｍｌ之间线性关系良好，相关系ｒ＝０．９９９９，平均回收率为１００．１％，ＲＳＤ＝０．５７％（。结论本方法简便，准确，重现性好。
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| 关 键 词： | 分光光度法 磷 大豆磷脂 测定 |
Determination of phosphorus level in soya lecithin by spectrophotometry |
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| Ma Jinling. Determination of phosphorus level in soya lecithin by spectrophotometry[J]. Heilongjiang Medicine and Pharmacy, 2000, 23(1): 21-21 |
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| Authors: | Ma Jinling |
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| Abstract: | Objective: To develop an assay for the determination of phosphorus level in soya lecithin by spectrophotometry Methods: Spectrophotometry was se lected to determine the phospho rus l e ve I in soya l e c ith in, based on the fo rmation of the blue complex by phosphate with ammonium molybdate and Fiske-Subbarow reagent in acid solution. Resulitsf The linearity for phosphorus was ranged over 0.04-0.16ug/ml with r=0.9999, the average recovery of 100. 1% and RSD of 0.57% (n=9). Conclusion The method was proved to be simple, precise and reproduciable. |
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| Keywords: | soya lecithin phosphorus spectrophotometry |
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