肝硬化大鼠肠道Cajal间质细胞的研究

目的:观察肝硬化大鼠肠道Cajal间质细胞的改变,探讨肝硬化胃肠动力障碍的相关机制.方法:Wistar大鼠20只随机分为肝硬化模型组和对照组,每组10只,采用CCl4溶剂大腿根部皮下注射法制作肝硬化模型,用葡聚糖蓝-2000为胃肠内标记物,观察大鼠肠道传输,免疫组化染色观察空肠c-kit阳性Cajal间质细胞的变化,并用透射电镜观察Cajal间质细胞超微结构的改变.结果:与对照组比较,肝硬化组大鼠小肠动力明显减弱(P<0.01),空肠c-kit阳性Cajal间质细胞减少(P<0.01),透射电镜观察显示Cajal间质细胞的超微结构发生明显改变.结论:肝硬化大鼠肠道传输减弱与肠道Cajal间质细胞减少及其超微结构改变有关.

Study of Cajal interstitial cells in intestine of rats with liver cirrhosis

Objective: To observe the changes of Cajal interstitial cells(CIC) in the intestine of rats with liver cirrhosis,so as to study the mechanism of gastrointestinal dysfunction.Methods: Twenty Wistar rats were equally randomized into control group and cirrhosis model group.Cirrhosis model was established by CCl_(4) in rats.The intestinal motility changes were assayed using Dextran blue-2000 as an indicator.Immunohistochemical staining was used to investigate the distribution of c-kit positive CIC in jejunum of rats.Meanwhile,the ultrastructure of CIC was observed by electron microscope.Results: Compared with control group,the intestinal motility and the c-kit positive CIC in jejunum were both markedly decreased in model group(both P<0.01).The CIC,with less organelles and dissovled cytoplasm,had decreased connections with other cells and damaged structure.Conclusion: The intestinal motility of rats with liver cirrhosis is significantly decreased,which may be associated with the changes of CIC number and ultrastructure in intestine.