灵芝多糖对糖尿病大鼠胸主动脉氧化应激的影响

目的：研究灵芝多糖对链脲佐菌素（STZ）诱导的糖尿病大鼠胸主动脉内皮损伤的保护作用及对糖尿病大鼠胸主动脉氧化应激的影响。方法：SD 大鼠经过4周高脂饮食后腹腔注射链脲佐菌素（30 mg·kg-1）造模，随机分为正常对照组、模型组、灵芝多糖（GLPs）高、低剂量组。经过12周给药治疗后，测定血清中过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）、总胆固醇（TC）和甘油三酯（TG）的水平；然后胸主动脉切片 HE 染色，观察胸主动脉超微结构及病理改变。结果：GLPs-H 组血糖浓度较模型组相比均明显下降（P<0.01），血清 CAT 和 GSH-PX 水平较模型组相比均明显上升（P<0.01）；血清 TC、TG 含量较模型组相比均明显下降（P<0.01）。 HE 染色，与模型组相比，GLPs-H 组光镜下主动脉内膜增厚、内皮损伤、中膜增厚的情况有所改善。结论：灵芝多糖可减轻糖尿病大鼠体内氧化应激水平，具有减轻脂质过氧化作用，对糖尿病胸主动脉并发症的发生发展有一定的预防作用。

Effect of Ganoderma Lucidum Polyccharide on Aorta Pectoralis Oxidative Stress

Objective: To investigate the protective effects of ganoderma lucidum polysaccharide (GLPs) on the impairment of the aorta pectoralis in the STZ induced diabetes mellitus (DM) rats and to explore the effects of GLPs on the oxidative stress in DM rats. Methods: SD rats were fed with high fat diet for 4 weeks, then injected STZ (30 mg·kg^-1) to replicate type 2 diabetic rats. The successful diabetic model rats were randomized into normal control group, diabetes group, Ganoderma lucidum polysaccharides of low and high-dose groups. After 12 week''s treatment, the activity of catalase (CAT) and glutathione peroxidase (GSH-PX), the levels of total cholesterol (TC) and triglycerides (TG) in serum were detected. Slice of aorta pectoralis were stained with HE to observe ultramicro structure and patho-change of aorta pectoralis. Results: The blood glucose concentration of the GLPs-H group was lower than that of the diabetes group (P<0.01); the activities of catalase and glutathione peroxidase in serum were significantly higher in the GLPs-H group than in the diabetes group (P<0.01); The levels of TC and TG in serum were significantly lower in the GLPs-H group than in the diabetes group (P<0.01). When observed under light microscope for HE stains, compared with the DM group with aortic tunica intima incrassation, injury of endodermis and tunica media incrassation, the GLPs-H group showed less damages. Conclusion: There is a confirmed action of GLPs in depressing oxidative stress and lipid peroxidation of the diabetes rats, and GLPs play a role in the prevention of diabetic complications of aorta pectoralis.