| 热应激对大鼠肝脏节律性基因Per2mRNA表达水平的影响 |
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| 引用本文： | Li G,Li H,Zhang Q,Liu H,Liu H,Shido O. 热应激对大鼠肝脏节律性基因Per2mRNA表达水平的影响[J]. 南方医科大学学报, 2012, 32(2): 215-217 |
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| 作者姓名： | Li G Li H Zhang Q Liu H Liu H Shido O |
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| 作者单位： | 宁夏医科大学基础医学院生理学系;宁夏医科大学总医院;日本岛根大学医学部 |
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| 基金项目： | 国家自然科学基金(81060230);宁夏自然科学基金(NZ10111)~~ |
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| 摘 要： | 目的探讨急性热应激对大鼠节律基因Per2mRNA昼夜表达水平的影响。方法选用雄性Wistar大鼠,随机分为对照组和热暴露组。对照组:置于室温24℃环境中;热暴露组:环境温度为32℃,经7 d热暴露后,采用体温遥感技术监测大鼠体温变化,测定大鼠脑垂体与肾上腺的相对质量及肝脏Per2 mRNA昼夜的表达水平。结果急性热应激对大鼠体温和体质量无影响,但可增加大鼠脑垂体和肾上腺的相对质量,并可显著抑制节律基因Per2 mRNA在夜间的表达水平。结论急性热应激可干扰Per2 mRAN的昼夜节律性表达。
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| 关 键 词： | 热应激 脑垂体 肾上腺 肝脏 Per2 |
Effects of heat stress on Per2 mRNA expression in the liver of rats |
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| Li Guanghua,Li Hong,Zhang Qinghua,Liu Hongmei,Liu Hongpeng,Shido Osamu. Effects of heat stress on Per2 mRNA expression in the liver of rats[J]. Journal of Southern Medical University, 2012, 32(2): 215-217 |
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| Authors: | Li Guanghua Li Hong Zhang Qinghua Liu Hongmei Liu Hongpeng Shido Osamu |
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| Affiliation: | Department of Physiology, Ningxia Medical University, Yinchuan, China. ghlee0404@163.com |
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| Abstract: | Objective To investigate the effect of acute heat stress on the day-night circadian gene Per2 mRNA expression in the liver of rats.Method Male Wistar rats were randomly divided into two groups and exposed to heat at 32℃or to a room temperature at 24℃(control).After 7 days of heat exposure,the body temperature was measured by telemetry.The relative weight of the pituitary and adrenal glands was determined after the exposure,and liver Per2 mRNA expression level was detected using RT-PCR.Results Acute heat stress did not obviously affect body temperature or body weight of the rats.Seven days of heat exposure increased the relative weight of the pituitary and adrenal glands and significantly lowered Per2 mRNA expression level at night.Conclusion Acute heat stress can interfere with the day-night circadian gene Per2 mRNA expression in rats. |
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| Keywords: | heat stress pituitary adrenal gland liver Per2 |
| 本文献已被 CNKI PubMed 等数据库收录！ |
