| 胶质瘤中hsa-miR-20a的表达及对细胞增殖的影响 |
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| 引用本文： | Yao X,Li W,Wang Y,Ding Z,Hou W,Zeng M,Deng G,Zhang J,Yang H. 胶质瘤中hsa-miR-20a的表达及对细胞增殖的影响[J]. 南方医科大学学报, 2012, 32(2): 198-201 |
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| 作者姓名： | Yao X Li W Wang Y Ding Z Hou W Zeng M Deng G Zhang J Yang H |
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| 作者单位： | 清远市人民医院神经外科;吉林大学病理生物学教育部重点实验室;吉林大学第一医院神经外科 |
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| 基金项目： | 国家自然科学基金(30870970);吉林省科技发展计划项目(200905135)~~ |
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| 摘 要： | 目的探讨hsa-miR-20在胶质瘤中的表达情况及对胶质瘤细胞增殖的影响。方法应用实时荧光定量逆转录聚合酶链反应方法检测正常脑组织和不同级别脑胶质瘤组织中miR-20a的表达情况;脂质体转染miR-20a模拟物后,通过MTT比色法及流式细胞术观察胶质瘤U251细胞增殖的变化。结果 (1)与正常脑组织相比,胶质瘤组织miR-20a的表达水平显著增高(P=0.035);(2)胶质瘤组织恶性程度越高,miR-20a表达越高(P<0.001);(3)u251细胞转染miR-20a模拟物后,增殖能力明显加快,S期细胞比例明显增多(P<0.001)。结论 miR-20a能促进胶质瘤细胞的增殖,其高表达在脑胶质瘤的发生、发展中起重要作用。
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| 关 键 词： | 胶质瘤 hsa-miR-20a 细胞增殖 |
Expression of hsa-miR-20a in human glioma tissues and its effect on the proliferation of human glioma cells in vitro |
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| Yao Xingjun,Li Wei,Wang Yuehua,Ding Zhengbin,Hou Wenzhong,Zeng Minmin,Deng Guangce,Zhang Jinzhu,Yang Hongfa. Expression of hsa-miR-20a in human glioma tissues and its effect on the proliferation of human glioma cells in vitro[J]. Journal of Southern Medical University, 2012, 32(2): 198-201 |
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| Authors: | Yao Xingjun Li Wei Wang Yuehua Ding Zhengbin Hou Wenzhong Zeng Minmin Deng Guangce Zhang Jinzhu Yang Hongfa |
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| Affiliation: | Department of Neurosurgery, Qingyuan People's Hospital, Qingyuan, China. |
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| Abstract: | Objective To investigate miR-20a expression in human glioma and normal brain tissues and its effect on the proliferation of glioma cells in vitro.Methods The expression of miR-20a was detected in human normal brain tissues and glioma tissues by real-time RT-PCR.miR-20a mimics were synthesized and transfected into U251 cells via liposome,and the cell proliferation were detected using MTT assay and flow cytometry.Results The glioma tissues showed significantly up-regulated expression of miR-20a compared with normal brain tissues(P=0.035).The expression level of miR-20a was higher in high-grade than in low-grade gliomas.miR-20a mimics significantly enhanced the proliferation of U251 cells and the percentage of S-phase cells.Conclusion miR-20a shows potent effect in promoting the growth of glioma cells,suggesting its important role in the pathogenesis of human glioma. |
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| Keywords: | glioma hsa-miR-20a cell proliferation |
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