染料木黄酮通过上调血红素氧合酶-1表达减缓野百合碱诱导的大鼠肺动脉高压

目的研究染料木黄酮对野百合碱诱导的肺动脉高压大鼠肺组织血红素氧合酶-1(HO-1)表达的影响,探讨其减缓肺动脉高压的机制。方法 60只雄性SD大鼠随机分成对照组、野百合碱组、小剂量染料木黄酮治疗组和大剂量染料木黄酮治疗组。监测血流动力学变化,通过电子显微镜观察肺小动脉结构重塑,Western blotting检测肺组织HO-1的表达。结果染料木黄酮治疗组较野百合碱组大鼠平均肺动脉压降低(P<0.01),右心肥厚指数下调(P<0.01),大剂量组更显著(P<0.01)。染料木黄酮治疗组较野百合碱组大鼠肺组织HO-1表达上调(P<0.01),大剂量组更显著(P<0.01)。结论染料木黄酮能减缓野百合碱诱导的大鼠肺动脉高压,可能与其上调大鼠肺组织HO-1表达有关。

Genistein attenuates monocrotaline-induced pulmonary arterial hypertension in rats by up-regulating heme oxygenase-1 expression

Objective To study the effect of genistein on the expression of heme oxygenase-1(HO-1) in rats with pulmonary arterial hypertension(PAH) induced by monocrotaline(MCT).Methods Sixty male Sprague-Dawley rats were randomly divided into 4 groups(n=15),namely the control group,model group,low-dose(20μg/kg) genistein group and high-dose(80μg/kg) genistein group.The hemodynamic parameters were measured and the remodeling of pulmonary small arteries was observed by electron microscope(EM).The expression of HO-1 in the lung tissues were detected by Western blotting.Results Compared with the model group,genistein treatment significantly reduced the elevated mean pulmonary arterial pressure, improved the right ventricular hypertrophy index,and increased the expression of HO-1 in a dose-dependent manner. Conclusion Genistein attentuates pulmonry arterial hypertension in MCT-treated rats possibly by up-regulation of HO-1 in the lung tissues.