丹参注射液对冻融小鼠卵巢移植早期光镜结构及超微结构的影响

目的 探讨丹参注射液对冻融小鼠卵巢同种异体移植早期光镜结构及超微结构的影响.方法 收集1日龄小鼠卵巢,慢冻速融后移植至F1代8～12周雄鼠的肾被膜下,分别于移植后第2天(2d)、第7天(7d)和第14天(14d)回收两组卵巢移植物.光镜和电镜技术观察卵巢组织冻融前后和移植前后的组织形态学改变、计数卵泡数量.结果 小鼠卵巢经冻融后原始卵泡的存活率为87.9%.光、电镜观察卵巢组织移植后7d卵泡细胞和颗粒细胞结构欠完整,移植后14d卵泡结构恢复正常.移植后14d丹参组各级卵泡数及卵泡存活率(30.1%)多于生理盐水组(13.2%),差异显著(P＜0.05).结论 结合光镜和电镜观察能更全面地评价冻融过程和移植过程对小鼠卵巢组织的影响,移植早期的缺血缺氧对小鼠卵巢组织造成了远比冻融过程更严重的损伤;丹参注射液可能促进移植后冻融小鼠卵泡发育,减少细胞凋亡.

Effect of salvia miltiorrhiza on the histological structure and ultrastructure of mouse ovarian tissues frozen and thawed at early developmental stage after transplantation

Objective To investigate the effect of Salvia miltiorrhiza on the morphological structure and ultrastructure of mouse ovarian tissues frozen and thawed at the early developmental stage after transplantation. Methods The 1- day ovaries-thawed through cryopreservation treatment were xenografted into the donated kidney capsule of 8 to 12-week-male mice. The mice in two groups were sacrificed at day 2, 7 and 14 after transplantation respectively, and the ovaries transplanted were excised for observing histological structure and ultrastructure changes and for counting the ovarian follicles and detecting the apoptosis index of follicles. Results The survival rate of primordial follicles in the mouse ovaries-frozen and thawed was 87.9%. The histological structure and ultrastructure showed that both the follicles and stromata were damaged on day 7 after transplantation. The follicular structure had been recovered for 14 days. The number of follicles and survival rate after transplantation in Salvia miltiorrhiza group (30.1%) were higher than that in saline group(13.2%) at 14-day(EM>P/EM><0.05). Conclusion The injury of the mouse ovarian tissues at the early stage is due to the ischemia and hypoxia rather than the cryopreservation. Salvia miltiorrhiza may provide benefits for folliculogensis and decrease the apoptosis index of follicles.