应用聚合酶链反应对有异常妊娠史妇女人巨细胞病毒感染的探讨

应用聚合酶链反应（ＰＣＲ）对于有异常妊娠史妇女６０例，检测了血液、尿液和宫颈分泌物中的人巨细胞病毒（ＨＣＭＶ）ＤＮＡ；用ＥＬＩＳＡ检测血清中的ＨＣＭＶ－ＩｇＭ抗体。结果表明：有死胎、畸胎妊娠史的妇女，血、尿和宫颈分泌物中的ＨＣＭＶ－ＤＮＡ以及ＨＣＭＶ－ＩｇＭ阳性率分别为４２．８５％（１２／２８）、２８．５７％（８／２８）、５３．５７％（１５／２８）和２５．００％（７／２８）；习惯性流产史的妇女分别为２１．８７％（７／３２）、１２．５％（４／３２）、３１．２５％（１０／３２）和１５．６２（５／３２）；正常对照为１４．２８％（５／３５）、１１．４２％（４／３５）、１７．１４％（６／３５）和６．７１％（２／３５）。统计表明：畸胎、死胎组的血、尿和宫颈分泌物中的ＨＣＭＶ－ＤＮＡ明显高于对照Ｐ＜０．０５，有显著差异。习惯性流产组的四项指标与对照比较均无差异Ｐ＞０．０５。结果提示，血清中的ＨＣＭＶ－ＩｇＭ的阳性率低于血液和宫颈分泌物中的阳性率，而接近于尿中的结果。不同部位的标本，以宫颈分泌物的阳性率最高，其次是血液，再其次是尿液。

Study of BCMV infection with history of abnormal pregnancy by polymerase chain reaction

HCMV-DNA were detected in sample of blood, urine and cervical swab from 60 abnormal pregnant women by PCR, and HCMV-IgM in serum by ELISA, respectively.The study showed that the positive rates of HCMV-DNA with death and teratu fetal were 42. 85% (blood) and 28. 57% (urine) and 53.37%(cervical),and HCMV - IgM in serum was 25%,HCMV-DNA with aborted history were 21. 8% (blood), 12. 5% (urine) and 31.25% (cervical),and HCMV-IgM was 15.62% (serum).Compared with normal pregnancy 14. 28% (blood), 11. 42% (urine),17.14% (cervical), the positive rate of HCMV-DNA in sample of urine and cervical from women with history of death fetal and teratu were higher than that.The results suggested that women with history of death fetal and teratu was associated with HCMV infection, and the cervical was a important port of body where was more easy infected with HCMV.