T cells migrate to tumour sites after extracorporeal interleukin 2 stimulation and reinfusion in a patient with metastatic melanoma |
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| Authors: | R DUMMER JC BECKER C EILLES E SCHÄFER W BÖRNER G BURG |
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| Institution: | Department of Dermatology, University Hospital Zürtch, Gloriastr, 31, CH-8091 Zürich, Switzerland;Department of Nuclear Medicine, University of Würzburg, Josef-Schneider Str. 2, D-8700 Würzburg, Germany;Department of Dermatology, University of Würzburg, Josef-Schneider Str. 2, D-8700 Würzburg, Germany |
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| Abstract: | Peripheral blood mononuclear cells (PBMC) were taken by leukapheresis from a patient with melanoma skin metastases and stimulated in vitro using 1000 IU recombinant interleukin 2 (IL-2)/ml to generate lymphokine-activated killer cells (LAK cells). Two-colour immunofluorescence analysis demonstrated an IL-2-induced up-regulation of CD25 on natural killer cells (CD56+) as well as on T lymphocytes (CD3+). After radiolabelling with indium-111, the cells were reinfuse. Gamma-camera imaging revealed an enrichment at the tumour sites. Immunostaining of tumour tissue taken before and after scintigraphy demonstrated CD25+ Tlymphocytes (CD2+, CD3+), but no natural killer cells (CD16+, CD56+) infiltrating the metastases. LAK cell enrichment at melanoma metastases in vivo did not involve natural killer cells, but was characterized by increased numbers of activated T lymphocytes in this patient. |
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