地奥司明对肾缺血／再灌注大鼠肾组织PECAM-1表达的影响

摘要：目的观察地奥司明（diosmin，DOSM）对肾缺血／再灌注（ischemia／reperfusion，I／R）大鼠肾组织PECAM一1表达水平的影响，并探讨其对肾脏的保护机制。方法150只SD大鼠随机分成3组：假手术（shamoperation，SO）组、肾缺血／再灌注模型组（I／R）和地奥司明＋肾缺血／再灌注模型组（DOSM＋I／R）。在再灌注不同时间点分别采用免疫组化及Western免疫印迹方法测定肾脏组织中PECAM-1的表达水平。结果在1h、6h、12h、24h和48h不同时间点的肾组织中，I／R和DOSM＋I／R两组PECAM-1的表达水平均随时间逐渐上调，并于48h达最高。而在DOSM组显著低于I／R组俨〈0．05或P〈0．01）。Western免疫印迹与免疫组织化学检测结果基本一致。结论DOSM可显著降低肾I／R病理过程中PECAM-1的表达，有助于减轻I／R时炎性细胞的浸润，从而减轻缺血再灌注损伤，保护肾功能。

Effect of Diosmin on expression of PECAM-I in kidney tissue of rats following ischemia/reper- fusion injury

Objective To study the effect of diosmin （DOSM） on expression of PECAM-1 in kidney tissue of rats following ischemia/reperfusion （I/R） injury and its kidney protection mechanism. Methods One hundred and fifty SD rats were randomly divided into sham operation （SO） group, I/R group, and DOSM ＋I/R group. Expression level of PECAM-I in kidney tissue was measured by immunohistochemistry and Western blot, respectively, at different time points of I/R. Results The expression level of PECAM-1 in kidney tissue increased gradually at 1, 6, 12, 24, and 48 h in UR group and DOSM＋I/R group, reached its peak at 48 h, and was significantly lower in DOSM ＋I/R group than in I/R group （P 〈 0.01, P 〈 0.05）. No significant difference was found in the expression level of PECAM-1 measured by Western blot and immunohistochemistry. Conclusion DOSM can significantly down- regulate the expression of PECAM-1 in rats following I/R injury and alleviate the infiltration of inflammatory cells, thus improving I/R injury and protecting renal function.