骨髓干细胞参与小鼠急性肾小管坏死修复的实验研究

【摘要】 目的 观察在生理和病理情况下,骨髓来源的干细胞(BMSC)能否分化成肾小管上皮细胞。 方法 绿色荧光蛋白(GFP)标记的C57BL/6转基因小鼠提供骨髓细胞。同种无荧光标记的C57BL/6小鼠100只分为正常对照组、全身照射组、缺血再灌注组、骨髓移植组、骨髓移植+缺血再灌注组。受体鼠的骨髓重建经血液常规检查和流式细胞仪检测确认,并采用荧光组织化学、免疫组织化学等方法观察绿色荧光标记的BMSC在受体鼠肾脏的分布及数量。 结果 全身致死剂量γ射线照射未造成小鼠肾脏组织结构和生理功能的明显改变。骨髓移植后第56、84天的受体鼠肾小管中有少量GFP阳性细胞的存在(78.75±5.99)%、(79.58±4.60)%],激光共聚焦显微镜进一步证实这些细胞位于肾小管,并表达肾小管上皮细胞特异性的功能蛋白megalin。 结论 在生理和病理情况下,骨髓干细胞均可以向肾小管上皮细胞转分化,参与肾小管上皮细胞的更新,并且在急性肾小管坏死的病理条件下,骨髓干细胞的肾向转化率与肾脏受损程度有关。

Empirical study of bone marrow stem cells participating in the recovery of acute tubular necrosis in mice

【Abstract】 Objective To observe whether bone marrow stem cells can differentiate into renal tubular epithelial cells in physiology and pathology conditions. Methods Transgenic C57BL/6 mice labelled with green flouresent protein(GFP) were bone marrow donors. C57BL/6 mice without fluorescence label were divided into normal group, total body irradiation group,ischemia/reperfusion (IR) group, bone marrow transplantation (BMT) group, BMT+IR group. Bone marrow reconstruction of the recipients was proved by blood routine and flow cytometry of bone marrow cells after transplantation, and the distribution and the amount of green fluorescence in kidneys of recipients were observed by fluorescence histochemistry and immunohistochemistry. Results Lethally total body irradiation made no significant change in renal histological structure and function. Green fluorescence was seen in renal tubular epithelium of the recipients on day 56 and 84(positive cell,78.75%±5.99%,79.54%±4.60% after bone marrow transplantation. These positive cells locating in tubular epithelium were further proved by laser scanning confocal microscope, and fluorescence histochemistry showed these cells expressed renal tubular epithelium specific protein megalin. Conclusion Bone marrow stem cells can differentiate into renal tubular epithelial cells and participate in regeneration of renal tubular epithelium in physiology and pathology conditions, and the number of epithelial cells derived from the bone marrow is associated with the degree of the injury in acute tubular necrosis.