顶体形态与精子活力、活率关系分析

目的探讨精子顶体形态与精子活力、活率关系.方法123例男性不育患者,采用精子自动分析质量检测系统检测精液常规,采用伊红Y水实验分析精子活率,采用联苯胺染色计数精液白细胞浓度,用改良巴氏染色法分析精子形态及顶体形态.结果精子活率正常组顶体完整率明显高于精子活率异常组(P＜0.05);精子活力正常组与精子活力异常组顶体完整率比较差异无显著性(P＞0.05);精液参数正常组顶体完整率明显高于精液参数异常组(P＜0.05);顶体完整率与正常形态精子百分率、精子活率呈显著正相关关系(P＜0.05,P＜0.01),与白细胞浓度呈显著负相关关系(P＜0.05).结论顶体形态分析可以在一定程度上反映男性生育力,是男性不育症诊断的一个有价值指标.

Correlation between acrosomal morphology and sperm motility, viability

Objective] To investigate the relationship between sperm acrosomal morphology and sperm motility,viability.Methods] Human semen samples were collected from 123 male patients with infertility.Semen parameters were assessed by using CASA.Sperm viability and semen leukocytospermia concentration were detected respectively by means of eosin Y stain and benzidine stain.Sperm morphology and acrosomal morphology were evaluated after modified papanicolaou stain.Results] The rate of intact acrosome in normal sperm viability group was significantly higher than that in abnormal sperm viability group(P <0.05).No significant differences were observed in the comparison between normal sperm motility group and abnormal sperm motility group(P >0.05).Compared with abnormal semen parameters group,the rate of intact acrosome was increased in normal semen parameters group(P <0.05).The rate of intact acrosome was positively correlated with the percentage of morphologically normal sperm and sperm viability(P <0.05,P <0.01),and negatively correlated with the semen leukocytospermia concentration(P <0.05).Conclusion] Sperm acrosomal morphology can reflect the potential of male fertility to some extent and can be regarded as a good predictor for diagnosis of male infertility.