消渴灵浓缩液对糖尿病大鼠坐骨神经IGF-1 mRNA表达的影响

目的：探讨消渴灵浓缩液对糖尿病大鼠坐骨神经IGF-1 mRNA表达的影响。方法：将30只链脲佐菌素诱导的糖尿病大鼠模型随机分为模型组、西药对照组、中药治疗组，每组10只。另选10只鼠龄、体重相匹配的大鼠作为正常组。采用相对定量逆转录聚合酶链式反应（RT-PCR）检测病程8周后各组大鼠坐骨神经IGF-1 mRNA表达的水平。结果：中药组、西药组、正常大鼠组坐骨神经IGF-1 mRNA相对表达量均增加，差异无统计学意义（P=0.213，P=0.822，P=0.304）；模型组坐骨神经IGF-1 mRNA相对表达量下降，与中药组、西药组、正常大鼠组比较，差异均有统计学意义（P<0.05）。IGF-1 mRNA表达水平与血糖呈负相关（P<0.05）。结论：糖尿病大鼠坐骨神经组织IGF-1 mRNA的表达下降，消渴灵浓缩液可上调糖尿病大鼠坐骨神经IGF-1 mRNA的表达水平，可能是消渴灵浓缩液防治糖尿病周围神经病变的作用机制之一。

Effect of xiaokeling concentration fluid on mRNA expression of insulin-like growth factor-1 in sciatic nerve of Streptozotocin-induced diabetic rats

Objective To investigate the effect of xiaokeling concentration fluid on insulin-like growth factor-1(IGF-1) mRNA expression in sciatic nerve of Streptozotocin-induced diabetic rats. Methods Thirty diabetic rats were randomly divided into model group, mecobalamin tablets group, and xiaokeling concentration fluid group. The IGF-1 mRNA level in sciatic nerve of each group was determined after 8 weeks by relative quantity RT-PCR. Results The IGF-1 mRNA level in sciatic nerve of diabetic rats between xiaokeling concentration fluid group, mecobalamin tablets group and normal group showed no significant difference (P=0.213,P=0.822,P=0.304), while was significantly higher than that of the model group (P<0.05). IGF-1 mRNA level was negatively correlated with the level of blood sugar (P<0.05). Conclusion IGF-1 mRNA level decreased in sciatic nerve of diabetic rats. Xiaokeling concentration fluid can increase the IGF-1 mRNA level in sciatic nerve of diabetic rats. Xiaokeling concentration fluid is involved in the regulation of IGF-1 expression, and probably prevents diabetic peripheral neuropathy from deteriorating.