GC法同时测定肉豆蔻中丁香酚、异丁香酚、肉豆蔻醚和榄香脂素的含量

目的:建立同时测定肉豆蔻中丁香酚、异丁香酚、肉豆蔻醚和榄香脂素4个成分含量的气相色谱法。方法:样品用乙酸乙酯超声提取,采用GC法对肉豆蔻中丁香酚、异丁香酚、肉豆蔻醚和榄香脂素进行含量测定。气相色谱条件:HP-5 MS石英毛细管柱(0.25 mm×30 m×0.25μm);氢火焰离子化检测器(FID);进样口温度240℃;检测器温度250℃;柱温起始温度为40℃,保持1 min,以5℃.min-1的速率升高到220℃,保持1 min;载气为氮气,流速1 mL.min-1,分流比为10∶1;进样量1μL。结果:丁香酚、异丁香酚、肉豆蔻醚和榄香脂素4个成分在相应的浓度范围内呈现良好的线性关系;加样回收率(n=9)分别为99.4%,99.3%,98.2%,98.8%;肉豆蔻不同部位中丁香酚、异丁香酚、肉豆蔻醚和榄香脂素的含量有明显差异。结论:本法简便快速,可以用于肉豆蔻中丁香酚、异丁香酚、肉豆蔻醚和榄香脂素4个成分的含量测定。

Simultaneous determination of eugenol,isoeugenol,myristicin and elemicin in Myristica fragrans Houtt. by GC

Objective:To establish a GC method for simultaneous determination of four components(eugenol,isoeugenol,myristicin,elemicin) in Myristica fragrans.Methods: Samples were extracted with ethyl acetate using supersonic fluid extraction.GC method was applied for the determination of eugenol,isoeugenol,myristicin and elemicin in Myristica fragrans.Chromatographic conditions: HP-5MS capillary column(0.25 mm×30 m×0.25 μm) was adopted;the detector was FID;the inlet temperature was 240 ℃;the detector temperature was 250 ℃;the column temperature was programmed to start at 40 ℃,rising to 220 ℃ at a rate of 5 ℃·min-1.Nitrogen(1 mL·min-1) was used as the carrying gas,and the split ratio was 10∶ 1;the injection volume was 1 μL.Results: Four components(eugenol,isoeugenol,myristicin,elemicin) had good linear relationship in the corresponding concentration ranges.The average recoveries(n=9) of the above four components were 99.4%,99.3%,98.2%,and 98.8%,respectively.The contents of four components in different parts of Myristica fragrans were different.Conclusion: This method is simple,quick and specific,which can be applied for the simultaneous determination of eugenol,isoeugenol,myristicin,elemicin in Myristica fragrans.