| ICAM-1基因表达对大鼠急性重症胰腺炎肺损伤的影响 |
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| 引用本文： | 刘学民,徐军,刘青光,张梅,徐高峰,潘承恩. ICAM-1基因表达对大鼠急性重症胰腺炎肺损伤的影响[J]. 医学争鸣, 2004, 25(17): 1555-1557 |
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| 作者姓名： | 刘学民 徐军 刘青光 张梅 徐高峰 潘承恩 |
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| 作者单位： | 西安交通大学第一医院肝胆外科,陕西,西安,710061;西安交通大学第一医院肝胆外科,陕西,西安,710061;西安交通大学第一医院肝胆外科,陕西,西安,710061;西安交通大学第一医院肝胆外科,陕西,西安,710061;西安交通大学第一医院肝胆外科,陕西,西安,710061;西安交通大学第一医院肝胆外科,陕西,西安,710061 |
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| 基金项目： | 国家自然科学基金，西安交通大学校科研和教改项目 |
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| 摘 要： | 目的:探讨急性重症胰腺炎(ASP)大鼠肺组织细胞间黏附分子-1(ICAM-1)的基因表达及其与肺损伤的关系. 方法:将ASP大鼠分别在制模后1, 3, 5, 7和9 h点剖杀,取肺组织行病理观察、微血管通透性、组织含水量及髓过氧化物酶(MPO)活性测定,RT-PCR法检测肺组织ICAM-1基因表达. 结果:制模后肺组织ICAM-1基因表达在1 h后逐渐上升,至9 h达到峰值(P<0.05). 同时肺损伤程度逐渐加重,肺组织微血管通透性、组织含水量及MPO活性逐渐升高. 结论:ASP大鼠肺组织ICAM-1基因过度表达,且与肺损伤严重程度相关.
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| 关 键 词： | ICAM-1基因 胰腺炎 急性肺损伤 |
| 文章编号： | 1000-2790(2004)17-1555-03 |
| 修稿时间： | 2003-12-16 |
Influence of intrapulmonary expression of intercellular adhesion molecule-1 on acute lung injury following acute severe pancreatitis in rats |
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| Abstract: | AIM: To evaluate the intrapulmonary expression of ICAM 1 mRNA and its relationship with acute lung injury in acute severe pancreastitis (ASP). METHODS: Rats were sacrificed at 1, 3, 5, 7 and 9 h after inducing ASP model and lung tissues were obtained for pathological study, microvascular permeability, dry/wet ratio and MPO examination. Gene expression of ICAM 1 in lung tissue was detected by RT PCR. RESULTS: After inducing ASP model, gene expression of ICAM 1 in the lung rose gradually after 1 h and reached the peak at 9 h ( P <0.05), accompanied with gradual increase of microvascular permeability, dry/wet ratio and MPO activity. CONCLUSION: Severity of lung injury can be predicted by the overexpression of ICAM 1 gene in ASP rats. |
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| Keywords: | ICAM 1 gene pancreatitis acute lung injury |
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