|
|||||
|
|
Methylprednisolone induces activation of the contact system in a dose-dependent manner. An in vitro study |
|
| Authors: | Olav Roeise Jan H. Nuijens C. Erik Hack Bonno N. Bouma Jan O. Stadaas Ansgar O. Aasen |
| Affiliation: | a Department of Surgery and Institute for Experimental Medical Research, Ullevaal Hospital, University of Oslo, Norway b Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University Hospital Utrecht, Utrecht, The Netherlands c Univers-ity of Amsterdam, Amsterdam and Department of Hematology, University Hospital Utrecht, Utrecht, The Netherlands |
| Abstract: | The effect of methylprednisolone sodium succinate (MP) on the contact system of plasma was studied in human citrated pool plasma. Contact activation was demonstrated by the presence of plasma kallikrein (KK) activity and activated Hageman factor (FXIIa) and/or KK in complex with Cl inhibitor (Clinh), detected by chromogenic peptide substrates or radio immunoassays, using monoclonal antibodies directed to neodeterminants exposed on complexed Clinh, respectively. When plasma and different doses of MP were incubated for a period of 24 hours, the highest dose of MP (10 mg/ml) gave rapid and marked increases in KK activities and concentrations of Clinh complexes. MP at 5 mg/ml plasma also induced activation of the contact system, although this activation was less pronounced. Even the lower dose of MP (1 mg/ml), which is equivalent to doses used in humans, increased plasma concentrations of KK-Clinh complexes. In conclusion, this study shows that MP in a dose-dependent way activates the contact system of plasma. |
| Keywords: | Author Keywords: Prekallikrein kallikrein high molecular weight kininogen factor XII factor XIIa-Cl-inhibitor and kallikrein-Cl-inhibitor complexes methylprednisolone in vitro |
| 本文献已被 ScienceDirect 等数据库收录！ | |