气相色谱法测定加味藿香正气丸中百秋李醇的含量

目的建立加味藿香正气丸中百秋李醇的GC含量测定方法。方法色谱柱：ZB-WAX石英毛细管柱（30m×0．25mm×0．25μm）。以100％聚乙二醇为固定相；柱温采用程序升温：起始170℃．保持6min．40℃／min升至230℃，保持3．5min；进样口温度：250℃；检测器温度：250℃；载气为氮气，流速为0．5mL·min^-1；进样量1μL，分流比为100：1。结果百秋李醇在0．0141～1．041mg·mL^-1浓度范围内呈良好的线性关系，r=1．00000；平均回收率为98．36％，RSD=1．56％（n=6）。结论该方法简单、准确、稳定、重现性好，可作为加味蓉香正气丸的质量控制的方法之一。

Determination of patchoulialcohol in Jiawei Hhuoxiang Zhengqi pills by gas chromatography

OBJECTIVE To establish a method for the determination of patchoulialcohol in Jiawei Huoxiang Zhengqi pills by gas chromatography（GC）. METHODS ZB-WAX capillary column（30m×0.25mm×0.25μm） was used and 100% Polyethylene Glycol was used as the liquid phase. The increase of column temperature was controlled by programming：the initial temperature was 170℃ and maintained for 6 min, and then rose at 40℃ min up to 230℃ for 3.5 rain. The injector temperature and the detector temperature both were 250℃ .Nitrogen was used as carrier gas, The flow rate was 0.5mL·min^-1 and the injection volume was 1μL, split ratio was 100： 1. RESULTS A good linearity was obtained in the range of 0. 0141-1. 041 mg·mL^-1, r = 1. 00000. The average recovery was 98.36 % with a RSD of 1.56 % （n = 6） . CONCLUSION The method is simple, accurate, reproducible and can be used for the quality control of Jiawei Huoxiang Zhengqi pills.