SA脂质体介导血管抑素和／或内皮抑素基因治疗Lewis肺癌小鼠的实验研究

 目的 观察SA脂质体介导血管抑素和／或内皮抑素基因对Lewis肺癌小鼠移植瘤生长、转移的抑制作用。方法 建立C57BL／6j小鼠肺癌模型，30只荷瘤鼠随机分空白对照组，SA脂质体对照组，血管抑素基因（pAng）治疗组，内皮抑素基因（pEnd）治疗组，血管抑素和内皮抑素基因联合治疗组，每组6只。以SA脂质体介导，将血管抑素和／或内皮抑素基因直接注入移植瘤内，每周2次，共6周，每周测瘤体大小2次，6周末处死所有小鼠，观察瘤体大小变化、肺表面转移灶数、生存期等。结果 各治疗组均能抑制肿瘤增长及肺内转移，与对照组比较有统计学意义（P＜0.01），小鼠活动能力、饮食、对外界刺激的反应能力均无明显改变，生存期明显长于对照组。结论 SA脂质体介导血管抑素和／或内皮抑素基因治疗可有效地抑制Lewis肺癌移植瘤的生长、转移，生存期明显长于对照组。

Cationic liposomes complexed to plasmids encoding endostatin and angiostatin inhibit lewis lung cancer in mice model

Objective To evaluate the inhibitory effect of cationic Liposomes complexed to plasmids encoding endostatin and/or angiostatin on the growth and metastasis of Lewis lung cancer in mice model. Methods C57BL/6j mice were established as mice model. Cationic liposome complexed plasmids encoding angiostatin and endostatin were administrated intratumorally to inhibit the growth and metastasis of the implanted tumor. The size change of the tumor; metastasis in lung, the activity, nourishment, survival period of the mice were observed to evaluate the function of cationic liposome complexed plasmids. Results The treatment group could inhibit the growth and metastasis of the implanted tumor. Comparing with control group, they showed significance in tumor size, metastasis in lung and survival period of the mice (P < 0.01). Conclusion Administrate intratumorally cationic liposome complexed plasmids encoding angiostatin and endostatin can powerfully inhibit the growth and metastasis of implanted lewis lung cancer in mice model.