XT-4000i血液分析仪对幼稚粒细胞检出的探讨

目的 XT-4000i血液分析仪对外周血幼稚粒细胞（IG）检出与显微镜法检查的相关性评价及仪器检测正常临界值的设定。方法 XT-4000i血液分析仪对182份血标本采用白细胞分类检测通道（DIFF通道）检测幼稚细胞,并用显微镜人工计数IG。结果 XT-4000i血液分析仪和镜检法对IG检出的相关性r2为0.500 0,XT-4000i血液分析仪对IG%检测的敏感性为68.7%,特异性为79.7%,临界值〉1。正常组的IG检测人工镜检均未见幼稚粒细胞,仪器检测显示IG%范围为0~0.81（-x±3s）。结论当XT-4000i血液分析仪检测结果IG%≤1（或IG#≤0.1×103/μL）可认为是正常标本,当IG%〉1（或IG#〉0.1×103/μL）则为异常标本,应进行血涂片复查以明确诊断。

Investigation on the detection of immature granulocyte by XT-4000i automatic hematology analyzer

Objective To investigate the correlation between XT-4000i automatic hematology analysis and microscopy in the detection of immature granulocyte（IG）,and set the critical value of IG for healthy subjects.Methods The IG of 182 blood samples were detected by XT-4000i through differential（DIFF） channel,and the count of IG was calculated by manual microscopy.Results There was a correlation value between XT-4000i and microscope in the detection of IG among healthy subjects（r 2=0.5000）.In the determination of IG% by XT-4000i,the sensitivity was 68.7%,the specificity was 79.7%,and the critical value was 1.All samples of healthy group were found no IG by manual microscopy,and the IG% value range was from 0 to 0.81（±3s） by automatic hematology analysis.Conclusions When IG%≤1（or IG#≤0.1×103/μL） by XT-4000i,the sample can be regarded as normal.When IG%1（or IG#0.1×103/μL）,it can be regarded as abnormal,and blood smear should be used to recheck.