| 人端粒酶反转录酶启动子调控NK4基因靶向治疗结肠癌的实验研究 |
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| 引用本文： | 徐波,肖焕擎,曾山崎,黄桂填,刘振邦,钱跃军,李书华. 人端粒酶反转录酶启动子调控NK4基因靶向治疗结肠癌的实验研究[J]. 中华胃肠外科杂志, 2010, 13(11): 851-854. DOI: 10.3760/cma.j.issn.1671-0274.2010.11.020 |
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| 作者姓名： | 徐波 肖焕擎 曾山崎 黄桂填 刘振邦 钱跃军 李书华 |
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| 作者单位： | 1. 广州医学院附属广州市第一人民医院普通外科,510180
2. 广州医学院附属广州市第一人民医院病理教研室,510180 |
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| 基金项目： | 广东省科技计划项目，广州市医药卫生科技项目 |
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| 摘 要： | 目的 构建人端粒酶反转录酶（HTERT）启动子调控的NK4基因重组腺病毒表达系统,探讨NK4基因对结肠癌生长、侵袭转移的抑制作用.方法 以Ad HTERTp-NK4重组腺病毒载体感染结肠癌细胞HTC116,分别以RT-PCR方法和Western blot检测细胞内NK4基因mRNA及蛋白表达量：MTT和体外侵袭实验观察NK4对结肠癌细胞增殖及侵袭转移能力的抑制作用.建立结肠癌裸鼠皮下移植瘤模型,观察重组腺病毒Ad HTERTp-NK4对裸鼠结肠癌移植瘤生长的影响.结果 Ad HTERTp-NK4重组腺病毒载体感染HCT116细胞后,HCT116细胞内NK4 mRNA及蛋白表达水平明显增高：Ad HTERTp-NK4重组腺病毒感染后,HCT116细胞生长抑制率和侵袭抑制率分别为47.14%和40.63%,与Ad HTERTp-GFP（空载体）组（2.75%和2.31%）比较,差异均有统计学意义（均P＜0.05）.局部注射Ad HTERTp-NK4重组腺病毒载体后,结肠癌裸鼠皮下移植瘤模型肿瘤生长受抑,抑瘤率为47.3%,与空载体组（4.6%）比较,差异有统计学意义（P＜0.05）.结论 HTERT启动子调控NK4基因靶向治疗系统可有效抑制结肠癌的增殖及侵袭转移,在结肠癌的基因治疗具有较好的应用前景.
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| 关 键 词： | 结肠肿瘤 人端粒酶反转录酶启动子 基因,NK4 |
Experimental study of NK4 gene targeted therapy mediated by human telomerase reverse transcriptase promoter for colon cancer |
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| XU Bo,XIAO Huan-qing,ZENG Shan-qi,HUANG Gui-tian,LIU Zhen-bang,QIAN Yue-jun,LI Shu-hua. Experimental study of NK4 gene targeted therapy mediated by human telomerase reverse transcriptase promoter for colon cancer[J]. Chinese journal of gastrointestinal surgery, 2010, 13(11): 851-854. DOI: 10.3760/cma.j.issn.1671-0274.2010.11.020 |
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| Authors: | XU Bo XIAO Huan-qing ZENG Shan-qi HUANG Gui-tian LIU Zhen-bang QIAN Yue-jun LI Shu-hua |
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| Affiliation: | XU Bo(Department of General Surgery,The First Municipal People＇s Hospital of Guangzhou,Guangzhou Medical College,Guangzhou 510180,China) XIAO Huan-qing(Department of General Surgery,The First Municipal People＇s Hospital of Guangzhou,Guangzhou Medical College,Guangzhou 510180,China) ZENG Shan-qi(Department of General Surgery,The First Municipal People＇s Hospital of Guangzhou,Guangzhou Medical College,Guangzhou 510180,China) HUANG Gui-tian(Department of General Surgery,The First Municipal People＇s Hospital of Guangzhou,Guangzhou Medical College,Guangzhou 510180,China) LIU Zhen-bang(Department of General Surgery,The First Municipal People＇s Hospital of Guangzhou,Guangzhou Medical College,Guangzhou 510180,China) QIAN Yue-jun(Department of General Surgery,The First Municipal People＇s Hospital of Guangzhou,Guangzhou Medical College,Guangzhou 510180,China) LI Shu-hua |
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