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大鼠骨骼肌细胞葡萄糖—6磷酸酶活性的细胞化学研究
引用本文:王仁鹏,孙榆.大鼠骨骼肌细胞葡萄糖—6磷酸酶活性的细胞化学研究[J].第三军医大学学报,1992,14(1):22-25.
作者姓名:王仁鹏  孙榆
作者单位:第三军医大学训练部电镜室 630038 (王仁鹏),第三军医大学组胚教研室 630038 (孙榆),山东师范大学 进修生(李国荣)
摘    要:本文用酶细胞化学葡萄糖-6-磷酸酶(G6Pase)技术对大鼠骨骼肌细胞进行了超微结构研究,结果证实,骨骼肌细胞内含有G6Pase。G6Pase活性反应产物定位于肌浆网与核被膜内,在肌浆网内,该酶活性反应产物呈异质性分布。根据肌浆网与肌膜系统之间的位置关系,骨骼肌偶联有以下3种类型:(1)终池与横小管之间的偶联,如三联体;(2)周围肌浆网与表面肌膜之间的偶联,即周围偶联;(3)肌膜下肌浆网与肌膜下横小管之间的偶联,即肌膜下偶联。对本实验结果进行了讨论。

关 键 词:葡萄糖磷酸酶    超微结构  大鼠

Cytochemical study on glucose-6-phosphatase activity in skeletal muscle cells in rats
Wang Renpeng,Sun Yu,Li Guorong.Cytochemical study on glucose-6-phosphatase activity in skeletal muscle cells in rats[J].Acta Academiae Medicinae Militaris Tertiae,1992,14(1):22-25.
Authors:Wang Renpeng  Sun Yu  Li Guorong
Abstract:Glucose-6-phosphatase (G6Pase) activity in the skeletal muscle cells was observed with electron microscope in rats.It was found that the reaction product of G6Pase activity was localized in the sarcoplasmic reticulum (SR) and nuclear envelope and a heterogenous distribution of G6Pase activity in the SR was shown.On the basis of the position relationship between SR and sarcolemmal system,the skeletal couplings can be classified as follows.1.The coupling between the terminal cisternae and the transverse tubules such as a triad.2.The peripheral coupling between the peripheral SR and the surface sarcolemma.3.The coupling between the subsarcolemmal SR and the subsarcolemmaltransverse tubules.The significance of the findings was discussed.
Keywords:glucosephosphatase  muscle/UL  sarcoplasmic reticulum  rat
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