妊娠合并重症甲型H1N1流感患者T细胞亚群及Th1/Th2水平的变化

[目的]研究妊娠合并重症甲型H1N1流感患者T细胞亚群的分布特点及辅助性T细胞1/辅助性T细胞2(Th1/Th2)水平的变化.[方法]收集2009年11月14日至12月31日于中国医科大学附属盛京医院确诊的女性甲型H1N1流感患者34例,按是否妊娠分为甲流妊娠组(A组,17例),甲流非妊娠组(B组,17例),同时收集抗...

Changes of T-Cell Subsets and Th1/Th2 Levels in Pregnant Women with Severe Influenza A(H1N1)

[Objective] To study the distribution of T cell subsets and the level of Thl/Th2 cytokines inpregnant women with severe influenza A（H1N1）. [Methods]Thirty four female patients diagnosed as H1N1 virus infection during Nov. 14 to Dec. 31, 2009 were divided into pregnant patients with influenza（Group A, n =17） and non-pregnant patients with influenza（Group B, n = 17）. Another group of healthy pregnant female who had a positive anti-HIN1 IgG served as a control group（Group C, n =15）. The percentage of CD4^＋ and CD8^＋ T-cell subsets was determined by using flow cytometry. Enzyme-linked immunosorbnent assay （ELISA） was used to examine serum interferon-gamma （IFN-7）, interleukin-4 （IL-4） and interleukin-10 （ IL- l0） levels. [Results] The median percentage of CD8^＋ T-cell in group A was 35 % （22%-40%） which was sig nificantly higher than that in group B[29.5% （14 %-32% ）1 （ P 〈0.05）. The median percentage of CD4^＋ Tcell and CD4^＋/CD8^＋ in group A was 35%（19%-43%） and 1. 054（0.49-1.77）, respectively, which were lower than those in group B[45%（35%-54%） and 1. 535（1.27-3.14）], and there was significant difference （all P 〈0.05）. Compared with group B, IL 10 level in group A increased significantly（ P %0.05）. Compared with group C, IFN-7 and IL 10 levels in group A and group B increased significantly（all P〈0. 05）. [Conclusion] Temporary immunodeficiency may be found in pregnant women with severe influenza A（HIN1）. The immune pathological damage caused by influenza A（HIN1） may be attributed to the increased Thl/Th2 cytokines.