高同型半胱氨酸血症对孕鼠胚胎发育的影响

[目的]探讨高同型半胱氨酸血症(Hhe)对大鼠妊娠的影响.[方法]将30只SD孕鼠按同型半胱氨酸(HCY)浓度高低随机分为 高剂量组(H组)、低剂量组(L组)、对照组(C组),每组10只.从妊娠d7起,H组腹腔内注 HCY 200 mg/(kg·d),L组注射100 mg/(kg·d),C组腹腔内注射同等体积的生理盐水...

Influence of Hyperhomocysteinemia on Embryo Development of Pregnant Rats

[Objective] To explore the influence of hyperhomocysteinemia（HHE） on rat pregnancy. [Methods]According to the level of homocysteine（HCY）, 30 SD pregnant rats were randomly divided into high dose group（group H）, low dose group（group L） and control group（group C） with 10 in each. From d7 to d20 of pregnancy before uterine-incision delivery, group A was intraperitoneally injected with 200mg/（kg. d）, and group L was injected with 100mg/（kg. d）, and group C was injected the equal volume of normal saline. The number of young rats and the dead fetus were counted. The weight and the height of young rats were measured. High performance liquid chromatography and electrochemical method were used to detect plasma HCY levels of pregnant rats before and d20 of pregnancy. [Results]Plasma HCY levels of pregnant rats in group H, L and C at d20 of pregnancy were 30.47±1.12, 20.90±1.08 and 10.98±0.77umol/L respectively, which were higher than those before the pregnancy, and there were significant differences among groups （all P 〈0.01）. As the dose of HCY increased, the number, weight and height of young rats in group H and L decreased, and the rate of dead and malformation fetus increased. There were significant differences between group H or L and group C（all P 〈0.05）. There was also significant difference between group H and group L （ P 〈0.05）. [Conclusion] HHE may exert the toxic effect on embryo development of pregnant rats and there is dose-effect relationship, which mainly shows the decreasing of the number of young rats, growth retardation, malformation and dead fetus.