通管方对输卵管炎性阻塞性不孕大鼠模型EGFR、ICAM-1表达的影响

目的探讨通管方对输卵管炎性阻塞性不孕(FTIOI)大鼠模型输卵管上皮细胞表皮生长因子受体(EGFR)及细胞间粘附分子-l(ICAM-1)表达的影响。方法选用180只Wistar雌性大鼠为受试对象,随机抽取30只作为正常组后,以"混合菌接种法"建立FTIOI大鼠模型;造模成功后按体质量随机分为模型组24只、血府逐瘀组25只及通管方低、中、高剂量组各24只,每组又分为15 d、30 d两个时间组。结果 (1)EGFR蛋白在正常大鼠输卵管上皮细胞阳性表达少量,模型组的EGFR蛋白表达增强明显,与正常组比较差异有显著统计学意义(P<0.01)。(2)ICAM-1蛋白在正常组中未见有表达,模型组存在高表达,两组间比较差异有显著统计学意义(P<0.01)。结论 (1)通管方能改善FTIOI模型大鼠输卵管的结构和功能,提高受孕率。(2)通管方能抑制输卵管上皮细胞EGFR蛋白的过度表达及ICAM-1的蛋白表达。

The Influence of Tong Guan Fang on the EGFR and ICAM-1's Expression of the Fallopian Tube Inflammatory Obstructive Infertility Rat Models

Objective Exploringhe influence of the traditional Chinese medicine Tong Guan Fang on the expression of EGFR and ICAM-1 about the tubal epithelium cells of FTIOI rat models.Methods Choosing 180 Wistar female rats as the subjects and randomly extracting 30 female rats as the normal group,later using the mixed bacteria inoculation method to establish FTIOI rat models;After making modles success,rat models will be randomly divided into model group for 24 rats,therapeutic group for 25 rats,Tong Guan Fang high,medium and low dose group for every 24 rats.Each group is divided into two time groups for 15 days and 30 days.Results(1)EGFR protein has a small amount of positive expression in the normal rats’s tubal epithelium cells,EGFR protein’s expression obviously enhances in the model group.Compared with the normal group,the model group has significant statistical significance(P<0.01).(2)ICAM-1 protein has not expression in normal group,but in the model group it exists high expression,so the comparative differences have remarkable statistical significance between the two groups(P<0.01).Conclusion(1)Tong Guan Fang can improve the structure and function of the fallopian tube of FTIOI rat models,increase the conception rate.(2)Tong Guan Fang can inhibit the overexpression of the tubal epithelium cells EGFR protein and the expression ICAM-1 protein.