| 志贺菌福氏2a301菌株torA基因改变的研究 |
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| 引用本文： | 张晶波,叶长芸,李新军,白雪梅,吴龙飞,徐建国. 志贺菌福氏2a301菌株torA基因改变的研究[J]. 中华微生物学和免疫学杂志, 2005, 25(3): 173-175 |
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| 作者姓名： | 张晶波 叶长芸 李新军 白雪梅 吴龙飞 徐建国 |
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| 作者单位： | 1. 102206,北京,中国疾病预防控制中心传染病预防控制所腹泻病研究室,卫生部医学分子细菌学重点实验室
2. 法国科学院细菌生化研究室 |
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| 基金项目： | 科技部 973课题资助 (G1 9990 5 4 1 0 1 ) |
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| 摘 要： | 目的探讨志贺菌福氏2a301菌株torA基因的改变以及与TorA(trimethylamine N-oxide TMAO reduetase，三甲胺N-氧化物还原酶)转运相关的TAT分泌系统的存在。方法利用酶活性检测和Western blot方法来验证志贺菌福氏2a301测序菌株torA基因发生的碱基置换，并且利用正常大肠杆菌中torA基因的克隆转化来研究TAT分泌系统的存在。结果实验表明志贺菌福氏2a301菌株不具有TorA酶活性，不表达TorA蛋白，和序列分析的结果相符；但是，将torA基因克隆子转化入301菌株后，转化子获得了TorA酶活性，表达了TorA蛋白。结论在志贺菌福氏2a301菌株中torA基因发生了改变，但具有完整的TAT分泌途径，可以转运蛋白。
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| 关 键 词： | 志贺菌 菌株 福氏 基因改变 Western rA基因 分泌系统 N-氧化物 酶活性检测 TAT blot 分泌途径 基因克隆 序列分析 大肠杆菌 克隆转化 转运蛋白 A蛋白 to 转化子 三甲胺 还原酶 |
| 修稿时间： | 2004-07-22 |
The change of torA gene of Shigella flexneri 2a301 strain |
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| ZHANG Jing-bo,YE Chang-yun,LI Xin-jun,BAI Xue-mei,WU Long-fei,XU Jian-guo. The change of torA gene of Shigella flexneri 2a301 strain[J]. Chinese Journal of Microbiology and Immunology, 2005, 25(3): 173-175 |
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| Authors: | ZHANG Jing-bo YE Chang-yun LI Xin-jun BAI Xue-mei WU Long-fei XU Jian-guo |
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| Abstract: | Objective To study the change of torA gene and TAT secretion system related to TorA(trimethylamine N oxide TMAO reductase) translocation in Shigella flexneri 2a301 strain. Methods The detection of the enzyme activity and Western blot were used and transformed the clone of torA gene of E.coli into Shigella flexneri 2a301. Results No TMAO reductase activity and protein expression of TorA were observed in S.flexneri 2a301, that is also proved by sequence analysis. However, when S.flexneri 2a301 was obtained the cloned intact gene from E.coli by transformation, the enzyme activity was restored and protein expression of TorA was detected. Conclusion Our results showed that torA gene in S.flexneri 2a301 is changed, but the TAT system can translocate the protein. |
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| Keywords: | torA gene TorA TAT system |
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