穴位注射胞二磷胆碱对脑外伤大鼠学习记忆功能的影响

目的观察足三里穴位注射胞二磷胆碱疗法对脑外伤大鼠学习记忆功能的影响。方法采用改进的Feeney 法建立大鼠脑外伤模型。40 只大鼠随机分为假手术组、对照组、穴位注水组、腹腔给药组和穴位注药组，每组8 只。假手术组不造模，腹腔注射等体积生理盐水。其他组造模后，对照组：腹腔注射等体积生理盐水；穴位注水组：足三里穴注射等体积生理盐水；腹腔给药组：胞二磷胆碱500 mg/kg 腹腔注射；穴位注药组：胞二磷胆碱以生理盐水溶解为500 mg/ml，500 mg/kg 足三里穴注射。连续14 d。通过水迷宫实验和避暗实验测定大鼠学习记忆功能。结果在Morris 水迷宫实验中，除假手术组外，穴位注药组大鼠逃避潜伏期短于其他组(P<0.05)；在避暗实验中，穴位注药组大鼠学习记忆潜伏期较其他组长(P<0.05)，错误次数较少。结论穴位注射胞二磷胆碱能明显改善脑外伤大鼠的学习记忆功能。

Effects of Citicoline Acupuncture Point Injection on Learning and Memory Function of Rats with Traumatic Brain Injury

Objective To explore the effects of citicoline injection into Zusanli point (ST36) on learning and memory function of rats after traumatic brain injury (TBI). Methods The model was induced with the improved Feeney method. The rats were randomly divided into 5 groups: sham-operation group, control group, acupuncture point saline injection group, intraperitoneal drug injection group and acupuncture point drug injection group with 8 rats in each group. The rats in the acupuncture point drug or saline injection group were treated with acupuncture injection of citicoline or saline daily. The rats in the intraperitoneal drug injection group and control group were treated with intraperitoneal injection of citicoline or saline daily. The treatment continued for 14 d. The learning and memory function was evaluated with the Morris water maze test and passive avoidance test. Results Acupuncture point injection of citicoline can significantly shorten the escape latent period of TBI rats in Morris water tests and extend the latent period of learning and memory of TBI rats (P<0.05). Conclusion Acupuncture point injection is effective on the recovery of learning and memory function of rats after TBI.