卵巢早衰患者外周血CD4~+CD25~+Treg细胞的变化及意义

目的:探讨CD4+CD25+调节性T细胞(即CD4+CD25+Treg细胞)在卵巢早衰发病机制中的作用。方法:流式细胞仪定量检测卵巢早衰(premature ovarian failure,POF)患者、卵巢储备功能下降(diminished ovarian reserve,DOR)患者及健康对照组外周血CD4+T、CD8+T细胞及CD4+CD25+Treg细胞数量,应用3H-thymidine掺入法测定POF患者及对照组外周血CD4+CD25+Treg细胞对效应性T细胞的增殖抑制功能。结果:与对照组相比,POF患者及DOR患者CD4+CD25+Treg细胞比例降低(P<0.01)、POF患者CD4+T/CD8+T细胞比值增高(P<0.05),DOR患者CD4+T/CD8+T细胞比值无明显变化(P>0.05);POF患者免疫抑制功能无明显降低(P>0.05)。结论:CD4+CD25+Treg细胞比例降低与T细胞亚群失衡可能是POF的发病机制。

Changes and Significances of CD4+CD25+T Regulatory Cells in Patients with Premature Ovary Failure

Objective: To explore the role of T lymphocyte subsets and CD4+CD25+T regulatory cells(CD4+CD25+Treg cells) in the pathogenesis of premature ovary failure(POF).Methods: Flow-cytometry analysis of CD4+T,CD8+T cells and CD4+CD25+Treg cells was performed on fresh peripheral blood in patients with POF(n=26),diminished ovarian reserve(DOR,n=19) and control group,the suppression function of CD4+CD25+Treg cells in POF group and the control were detected by 3H-thymidine experiment.Results: Compared with the control,the percentages of CD4+CD25+Treg cells in POF group and DOR gruop were obviously reduced(P<0.01),and the CD4+T/CD8+T ratio in POF group was significantly increased(P<0.05);but in DOR group,there was no significant difference;however the suppression function of CD4+CD25+Treg cells in POF group was not significantly decreased(P>0.05).Conclusion: The decreased CD4+CD25+Treg cells and the imbalance of T lymphocyte subsets may play an important role in the pathogenesis of POF.