C-sis反义寡脱氧核苷酸抑制兔主动脉平滑肌细胞增殖的时效关系

为观察局部处理Ｃ－ｓｉｓ反义寡脱氧核苷酸对平滑肌细胞增殖及新生内膜肥厚的抑制作用，球囊导管损伤６０只新西兰白兔胸主动脉后，经导管局部处理每只兔１ｍｇ（２ｍＬ）Ｃ－ｓｉｓ反义或正义寡脱氧核苷酸或２ｍＬ生理盐水，于损伤后第３、７、１４天和第２８天，用免疫组织化学方法结合图象分析，评价损伤动脉内膜平滑肌细胞增殖及新生内膜改变。结果发现，Ｃ－ｓｉｓ反义寡脱氧核苷酸明显抑制内膜平滑肌细胞增殖，最高抑制作用出

Time-Effective Relation of Proliferation of Aortic Smooth Muscle Cells after Lo cal Delivery of C-sis Proto-oncogene Antisense Oligodeoxynucleotides in Injured Aorta of Rabbits

Aim To study the inhibitory effect of locally delivered c-sis proto-oncogene antisense oligodeoxynucleotide (ODN) on aortic smooth muscle cells (ASMC)proliferation and neointimal thickening. following balloon angioplasty in the rabbit aorta at differential time.Methods 60 male New Zealand, White Fabbits were treated with local delivery of c-sis antisense ODN,c-sis sense ODN, or 0. 9% salt solution via balloon catheter following aorta balloon injury, ASMC proliferation and area in neointima was assessed with immunohistochemistry and image analyzer at 3, 7, 14, 28 days after local delivery.Results ASMC proliferation in neointima were reduced 69. 8%. 59 %, 51. 9%, 47. 8% in c-sis antisense ODN-treated group compared to c-sis sense ODNtreated group at 3, 7, 14, 28 d following aortic injury respectively; and the inhibitory rate of neointimal hyperplasia was 48. 9%, 77. 8%, 72. 6%, 70. 8% at the same time respectively.Conclusion The locally deliveried C-sis antisense ODN inhibited ASMC proliferation and neointimal thickening following rabbit aortic injury, the inhibitory effect was the highest at 3 d and 7 d, then reduced slowly. and caused by antisense mechanism.