GM-CSF对类风湿性关节炎单核细胞MMP-2,MMP-9合成及CD147表达的调节作用

目的:观察类风湿性关节炎(RA)患者单核/巨噬细胞用GM-CSF刺激后,基质金属蛋白酶(MMP-2,MMP-9)合成及CD147表达的变化。方法:分离RA患者外周血及滑液单核细胞,用GM-CSF刺激,明胶酶谱法测定刺激后MMP-2,MMP-9的活性。用流式细胞术测定细胞表面CD147表达。结果:GM-CSF刺激后,RA单核细胞形态及表面标志改变,MMP-2,MMP-9合成及表面CD147增加,与GM-CSF的剂量相关;滑液单核细胞高于外周血。相关性分析证实CD147表达与MMP-2,MMP-9增加呈正相关。结论:GM-CSF可以促进单核/巨噬细胞CD147表达及MMP合成,这可能是RA发病中CD147表达及MMP合成的调控机制之一。

Stimulating effects of GM-CSF on MMP-2, MMP-9 production and CD147 expression by monocyte/macrophage of rheumatoid arthritis patients

Objective:To study the stimulating effects of GM-CSF on matrix metalloproteinases-2,9 production and CD147 expression by monocyte/macrophage from peripheral blood and synovial fluid of rheumatoid arthritis(patients).Methods: Monocytes/macrophage were isolated from peripheral blood and synovial fluid of rheumatoid arthritis patients with Ficoll-Hypaque,and was incubated in RPMI1640 with different amounts of GM-CSF.The activity and contents of MMP-2,MMP-9 was evaluated by Gelatin zymography.CD147 expressed on the surface of monocyte/macrophage was assessed by flow cytometry.Results:The production of MMP-2,MMP-9 and CD147 expression by monocyte/macrophage from peripheral blood and synovial fluids of RA patients was enhanced significantly when stimulated with GM-CSF,and the elevation was correlated with the dosage of GM-CSF.(Significant) correlation was found between CD147 expression and MMP-2,9 production.Conclusion:GM-CSF can stimulate MMP-2,MMP-9 production and CD147 expression by monocyte/macrophage and this may be one manipulating mechanisms of CD147 expression and MMP production in the pathogenesis of RA.