mdm2和P53在人脑胶质瘤中的相关性分析

目的　通过对人脑胶质瘤标本中 p5 3、mdm2蛋白表达关系的研究 ,探讨人脑胶质瘤的发病机理。 方法　应用免疫组化 (链霉素菌抗生物素蛋白 -过氧化酶法Streptavidin peroxidase ,S -P法 )标记检测 10 0例人脑胶质瘤标本 p5 3和mdm2 ,同时标记DNA原位杂交探针 p5 3。结果 10 0例脑胶质瘤标本 p5 3和mdm2免疫组化标记阳性率分别为 35 %、32 %;p5 3DNA探针原位杂交标记 ,阳性率为 90 %,其中 2 8例免疫组化标记mdm2阳性 ,而 p5 3阴性标本的原位杂交 p5 3阳性率为 89.2 8%(2 5例 )。统计学显示p5 3基因表达和mdm2基因表达有密切关系 (P <0 .0 5 )。结论　mdm2和 p5 3呈明显负相关 ,mdm2在人脑胶质瘤的发病过程中起重要的作用 ;p5 3的缺失不是人脑胶质瘤的主要发病机理 ,mdm2和 p5 3协同作用在脑胶质瘤的发生和发展中起重要作用。

The Correlation Between p53 and mdm2 in Human Gliomas

Objective To investigate the expression of p53 and mdm2 proteins in human glioma and analyze their correlation to figure out the formulation.Methods Immunohistochemical method and DNA in situ hybridization were used to detect the p53 and mdm2 gene expression in 100 cases of human glioma.Results Of the 100 cases Immunohistochemical positive rate of p53 and mdm2 were 35% and 32%,respectively,while DNA in situ hybridization positive rate of p53 was 90%. Especially,DNA in situ hybridization positive rate of p53,in 28 cases with Immunohistochemical negative p53 but positive mdm2,was 89.28%.The gene expression of p53 was related to that of mdm2(P<0.05).Conclusion The altered expression of oncogene mdm2 and antioncogene p53 might play a promotive role in the oncogenesis and development of human glioma.