Immunological evaluation of a 26-kDa antigen from Taenia solium larvae for specific immunodiagnosis of human neurocysticercosis |
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| Authors: | Lisiane V. Ev Antonio A. M. Maia Geraldo Pianetti E. Nascimento |
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| Affiliation: | (1) Department of Microbiology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil, BR;(2) Department of Neurology, Medical School, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil, BR;(3) Department of Parasitology/Laboratory of Molecular Biology of Parasites, Federal University of Minas Gerais, Av. Antonio Carlos 6627, Caixa Postal 482, `31270 901 Belo Horizonte, MG, Brazil E-mail: evaldo@mono.icb.ufmg.br, Fax: +55-31-441 6909, BR |
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| Abstract: | Human neurocysticercosis, due to infection of the central nervous system by cysticerci of Taenia solium, is a severe form of neurologic disease occurring in Central and South America. Specific proteins from scolex antigen from cysticerci were purified by polyacrylamide gel electrophoresis and electroelution and recognized in Western blots by antibodies present in sera from patients with neurocysticercosis. The proteins appeared as 13-, 17-, and 26-kDa bands on Coomassie blue-stained gels and proved to be specific to cysticerci of T. solium. No cross-reactivity with sera from patients with taeniasis or hydatidosis was observed. Enzyme-linked immunosorbent assay using the purified proteins of 13, 17, and 26 kDa demonstrated rates of 53%, 88%, and 100% specificity, respectively, at the cutoff serum dilution of 1:32 for the specific immunodiagnosis of␣human neurocysticercosis. Received: 10 May 1998 / Accepted: 21 July 1998 |
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