RecA蛋白-互补单链DNA探针提高压电基因传感器灵敏度的实验研究

目的研究利用RecA蛋白-互补单链DNA探针与固定的肽核酸(bis-PNA)探针相结合提高压电基因传感器的灵敏度的可行性。方法基因传感器阵列固定上针对乙肝病毒的bis-PNA探针,加入靶DNA与之反应,然后加入预先处理过的的RecA蛋白-互补单链DNA探针。结果直接加入靶DNA所引起的频率改变为15.83±7.31Hz,反应时间为45.16±12.87min,加入RecA蛋白-互补单链DNA探针(浓度为3.0mg/ml),所引起的频率改变最为明显,为112.16±12.7Hz。结论在反应体系中加入RecA蛋白-互补单链DNA探针,能有效地提高压电基因传感器的灵敏度,明显缩短反应体系时间。

The experimental study of sensitivity improvement of piezoelectricity gene sensor by the probe of RecA protein-complementary single strand DNA

Objective To improve the sensitivity of piezoelectric gene sensor by increasing ist surface load on the basis of the probe of RecA protein-complementary single strand DNA and investigate the optimal condition in liquid phase.Methods Bis-PNA probe for detecting HBV was immobilized on the surface of gene sensor array. Different concentrations of the RecA protein-complementary single strand DNA probes were added after the bis-PNA probes were hybridized with corresponding target DNA. Results When using target DNA probes directly the change of the frequency and the cost-time of reaction were obvious, they were 112.16±12.7Hz and 17.74±5.33min respectively.Conclusion The sensitivity of piezoelectricity gene sensor array is improved and the time of procedure is decreased significantly by using the probe of RecA protein-complementary single strand DNA in reaction system.