亚慢性氟中毒影响大鼠骨转换的动态分析

目的观察亚慢性氟中毒过程中骨密度及激素水平的变化情况，进一步探讨氟中毒的发病机制。方法将雄性wistar大鼠按体质量随机分成2组，氟化钠组每日饮用含氟150mg／L的氟化钠水溶液，正常对照组饮用蒸馏水，共饲养6个月，每月处死一批动物，测定血清及骨组织中的含氟量，光镜观察长骨干骺端的骨小梁密度，用放免方法检测血清中甲状旁腺激素(PTH)、降钙素(CT)、骨钙素(BGP)水平。结果成功的复制出了大鼠氟中毒的模型：随着染氟时间的延长，氟化钠组的骨小梁密度同对照组相比明显增加，PFH水平持续维持在高水平，与对照组比较差异有统计学意义，BGP水平从第2个月开始明显高于对照组，但两组的总体趋势均呈明显下降趋势．两组间CT水平未见明显差异。结论高剂量氟可以通过持续影响PTH和BGP水平调控骨转换过程。

Dynamic analysis of effects of subchronic fluorosis on bone turnover in rats

Objective To observe the changes of bone mineral density mass and h ormone in the progress of subchronic fluorosis and further explore the pathogene sis of fluorosis. Methods Male Wistar rats were randomly divided into two grou ps according to their body weights. The rats in the sodium floride group were gi ven 150 mg/L sodium floride in distilled drinking water, the rats in the normal control group were given distilled water. These rats were bred for six months, e very month some of whom were sacrificed. The contents of serum fluorine and bone fluorine were examined; the density of bone trabecula in metaphysis of thighbon e were observed under light microscope; the contents of parathormone(PTH), calci tonin(CT) and osteocalcin(BGP) were detected by radioimmunoassay. Results Alo ng with the time of fluorine exposing, the density of bone trabecula in the sodi um floride group was markedly higher than that in the normal control group; the content of PTH remained at high level and there was a significant statistical di fference between the sodium floride group and the control group. Although having a tendency to decline, BGP in the sodium floride group was higher than that in the normal control group beginning from the second month. However there was no s tatistically significant difference in the level of CT between the two groups. C onclusions Fluoride at high level continuously affects the progress of bone t urnover via PTH and BGP.