Enhancement of growth ofMycobacterium malmoense by acidic pH and pyruvate |
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Authors: | M. -L. Katila J. Mattila E. Brander |
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Affiliation: | (1) Department of Clinical Microbiology, Kuopio University Central Hospital, SF-70210 Kuopio, Finland;(2) Public Health Institute, Tuberculosis Laboratory, Mannerheimintie 166, SF-00300 Helsinki, Finland |
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Abstract: | The growth ofMycobacterium malmoense is dysgonic and slow on ordinary mycobacterium media. The effect of pH and pyruvate on the growth of ten strains was studied on a modification of Löwenstein-Jensen medium. Growth appeared sooner and was more abundant at pH<6.5. At pH 7 or higher, it was scarcely or not at all visible after six weeks of incubation. Pyruvate enhanced the growth of five strains that grew only poorly on glycerol-containing medium, even at acidic pH. The parallel use of both pyruvate and glycerol-containing media, pH 6 to 6.5, and an incubation period of seven weeks or longer are recommended for the isolation ofMycobacterium malmoense on Löwenstein-Jensen medium. |
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