苍白球在电针及兴奋尾壳核对脚内核自发放电影响中的作用

目的 :观察苍白球在电针及兴奋尾壳核对脚内核自发放电影响中的作用。方法 :实验在Wistar大鼠上进行 ,采用玻璃微电极细胞外记录的方法 ,观察毁损苍白球 (GP)前后 ,电针“阳陵泉”和“环跳”穴 (强度 2mA ,频率为 2Hz与 1 0 0Hz交替的疏密波 ,持续 5min)及兴奋尾壳核 (微量注射 0 .5mol/L谷氨酸 3 μL)对脚内核 (EP)神经元自发放电的影响。结果 :电针对EP神经元自发放电似有兴奋作用 ,而兴奋尾壳核对其影响不大。毁损GP后电针对EP神经元自发放电的兴奋作用略有增加 ,兴奋尾壳核对EP神经元的自发放电产生抑制作用。结论 :在正常状态下电针对EP神经元自发放电的兴奋作用部分受到GP的抑制 ,兴奋尾壳核通过GP对EP产生兴奋作用

Involvement of Rat's Globus Pallidus in the Effects of Electroacupuncture and Excitation of Caudate-putamen Nucleus on Spontaneous Discharges of Neurons in Entopeduncular Nucleus

Objective : To investigate the roles of rat's globus pallidus(GP) in the effects of electroacupuncture(EA) and excitation of caudate-putamen nucleus(CPu) on spontaneous discharges of neurons in entopeduncular nucleus(EP). Methods: The experiments were performed on 10% urethane-anesthetized (1 g/kg) Wistar rats. Glass microelectrodes were used for recording the spontaneous discharges of neurons in EP before and after chemical lesion (kainic acid, 10 μg/2 μL)of GP. All animals received EA of "Yanglingquan"(GB 34) and "Huantiao"(GB 30) with stimulating parameters of 2 mA of electric current, 2～100 Hz dense-sparse waves, and duration of 5 min. Excitation of neurons in CPu was achieved through microinjection of glutamate(0.5 M, 3 μL) into the nucleus. Results: It was found that the spontaneous discharges of neurons in EP were increased significantly by EA (6～8 min after EA, P<0.05) but not affected remarkably by exciting Cpu (P>0.05). In rats with lesion of globus pallidus, the spontaneous discharges of neurons in EP were increased slightly by EA and inhibited significantly by exciting Cpu (15 min and 20 min after administration of glutamate in comparison with saline group, P<0.05). Conclusion: Results suggest that the excitatory role of EA on the spontaneous discharges of EP neurons is partially inhibited by GP, and the activating CPu can induce an increase of the spontaneous discharges of EP neurons through GP.